摘要
目的采用UPLC-MS/MS和HPLC-UV测定肾移植患者麦考酚酸(MPA)血药浓度,考察方法间的相关性,建立普遍适用的测定结果换算关系。方法分别采用UPLC-MS/MS测定肾移植患者全血及血浆的麦考酚酸浓度,HPLC-UV测定肾移植患者血浆的麦考酚酸浓度,测定结果采用LEVENE和LSD法进行方差分析,用Spearman法进行相关性比较。结果经过统计分析得到3种方法互换方程式,分别为:Y质谱血浆=1.612 X质谱全血+0.686;Y液相血浆=1.414X质谱全血测定-0.206;Y液相血浆=0.848X质谱血浆-0.692。结论 UPLC-MS/MS测定肾移植患者全血及血浆麦考酚酸浓度与HPLC-UV测定肾移植患者血浆的麦考酚酸浓度,3种方法浓度结果间具有良好的相关性,通过方程式可进行结果互算。
OBJECTIVE To analyze the correlation between UPLC-MS/MS method and HPLC-UV method for determination of mycophenolie acid （MPA） blood concentrations in renal transplant patients and to establish a generally applicable conversion method of the determination results. METHODS MPA concentrations in whole blood and plasma of transplant patients were determined by UP- LC-MS/MS method. Plasma concentrations of MPA were determined by HPLC-UV. Variance analysis of the measure results was done by LEVENE and LSD methods, and the correlation between the methods was analyzed by Spearman method. RESULTS Three conversion formula were obtained as follows ： YuPLC MS/MS plasma = 1. 612 XUPLC-MS/MS whoLE hlood ＋ 0. 686, YHPLC-UV plasma = 1. 414XUPLC-MS/MS whole hlood - 0. 206, and YHPLC-UV plasma= 0. 848 XUPLC-MS/MS whole blood -0. 692. CONCLUSION There is good correlation between UPLC-MS/MS method and HPLC-UV method for the determination of MPA concentration in whole blood and plasma in renal transplant patients. The results can be transformed by equations.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2013年第6期461-465,共5页
Chinese Pharmaceutical Journal
基金
首都医学发展科研基金(2009-3021)
中日友好医院院级科研基金(2010-QN-29)
