摘要
目的观察HSP70／CD80DNA疫苗通过调节趋化因子及趋化因子受体对急性哮喘小鼠气道炎症和气道高反应性的治疗作用。方法以卵清蛋白（OVA）致敏小鼠，建立小鼠急性哮喘模型，同时肌肉注射HSP70／CD80DNA疫苗。观察小鼠气道反应性，外周血IgE，肺组织炎症和黏液分泌情况，CCL5和CCLl7在气道表达情况。Real—timePCR检测肺组织ccR5和CCR4基因表达。结果与对照小鼠相比，急性哮喘小鼠的气道反应性明显增高，血清IgE含量明显升高（P〈0．05），肺组织炎细胞浸润明显（P〈0．05），大量黏液形成。HSP70／CD80DNA疫苗治疗小鼠后，能有效减轻气道高反应性，降低血清IgE含量，降低抑制气道炎细胞浸润，减少黏液分泌（P〈0．05）；且CCL5在气道上皮呈阳性表达，CCLl7呈阴性（P〈0．05）；肺组织CCR5基因表达增加和CCR4表达减少（P〈0．05）。结论HSP70／CD80DNA疫苗可通过增强CCL5在气道上皮中的表达，减少CCLl7表达；上调CCR5抑制CCR4，使CCR5／CCR4升高，从而恢复Thl／Th2平衡，起到治疗哮喘的作用。
Objective To determine the effect of HSP70/CD80 DNA vaccine on airway inflamma- tion and airway hyperresponsiveness in asthma mice through regulating Thl/Th2 balance in the ehemokine and its receptor. Methods Mouse model of acute asthma was established by OVA-sensitized. At the same time, mice were injected with HSP70/CD80 DNA vaccine for observing airway responsiveness （ AR）, detec- ting serum IgE, measuring the pathological changes of lung tissue, and detecting CCL5 and CCL17 expres- sion. The expression levels of CCR5 and CCR4 mRNA in lung tissue were examined using real-time PCR. Results Compared with the control mice, mice with acute asthma showed a significant increase in airway reactivity and the infiltration of inflammatory cells in lung tissue. Mice treated with HSP70/CD80 DNA vac- cine could effectively reduce airway hyperresponsiveness in airway, as well as the perivascular inflammatory response （P〈0.05）. The expression of CCL5 was positive in airway epithelia, while CCL17 was negative （ P〈0.05 ）. CCR5 gene expression in lung tissue was increased, while CCR4 expression was decreased （ P〈 0.05）. Conclusion HSP70/CD80 DNA vaccine could treat asthma through restoring Thl/Th2 balance in two regulation ways, of which one was by enhancing the expression of CCL5 in airway epithelial cell and re- ducing CCL17 expression, the other was up-regulating the ratio of CCR5/CCR4 via increasing the expression of CCR5 and decreasing the expression of CCR4.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2013年第2期123-128,共6页
Chinese Journal of Microbiology and Immunology
基金
四川省科技厅应用基础研究项目（2009JY0076）
