摘要
低温富氮磷水体由于无明显水华现象,其潜在危害往往容易被人们所忽视。研究了一株耐冷反硝化土著菌Acinetobacter johnonii DBP-3对低温富营养化水体中典型营养盐的去除特征。结果表明,在避光、10℃和静置条件下培养,菌株对模拟系统中的氮磷和溶解性有机碳具有明显的去除作用,与氨氮相比,菌株优先利用硝酸盐氮。无底泥时,模拟系统培养7d后,硝酸盐氮、氨氮、溶解性磷酸盐和溶解性有机碳的浓度分别下降了65.7%、57.2%、62.4%和71.1%;底部覆盖底泥后,由于底泥向上覆水中释放了营养盐,导致水相中各营养盐的去除率有所降低,但水相中营养盐的浓度仍然低于无菌对照处理。整个实验周期内底泥中的总氮和总磷含量稍有下降,分别降低了6.5%和2.9%。菌株在模拟系统中能较好定殖,可保持数量上的优势,其中有一部分可以吸附于表层底泥,并保持活性。研究结果说明耐冷菌Acin-etobacter johnonii DBP-3在低温富营养化水体生物治理方面具有潜在的应用价值。
The potential hazard of water bodies rich in nitrogen and phosphorus at low temperatures is usually neglected due to no al gal blooms. This paper studies the removal of typical nutrients in eutrophic water bodies by a native psychrotolerant denitrifying bac- terium Acinetobacter johnonii DBP-3 in simulation systems. The results show that the addition of strain dBP-3 significantly increases the removal of nitrogen, phosphorus, and dissolved organic carbon under stationary cultivation conditions at 10 ℃ in darkness. In the simulation system without sludge, the concentrations of nitrate, ammonia, dissolve phosphate, and DOC decreases by 65. 7%, 57. 2%, 62. 4%, and 71.1 %, respectively, after 7 days＇cultivation. Moreover, strain DBP-3 gives priority to the utilization of ni- trate, as compared to ammonia. The addition of sludge slows down the removal of nutrients due to the release of nutrients into the upper water, whereas the concentrations of nutrients are still lower than that in the controlled treatment. During the entire experi- mental period, the content of total nitrogen and phosphorus in sediments decreases by 6. 5%0 and 2. 9%, respectively. The strain keeps numerical superiority in upper water and some of it is absorbed into the sediment during the whole experiment. It is indicated that the psychrotolerant Acinetobacter johnonii DBP-3 would be applicable for the biological treatment of eutrophic water bodies at low temperatures.
出处
《中国农村水利水电》
北大核心
2013年第2期8-11,共4页
China Rural Water and Hydropower
基金
国家自然基金(51109089)
吉林省科技发展计划项目(20090413
20100141)
吉林省教育厅十二五重点项目(201132)
关键词
富氮磷水体
生物强化修复
耐冷菌
低温脱氮除磷
water bodies rich in nitrogen and phosphorus
biological enhanced remediation
psychrotolerant bacterium
removal of nitrogen and phosphorus at low temperature
