摘要
目的:为了研究RPR与TP-ELISA检测方对产妇梅毒在临床上的检测价值。方法:对3479例产妇,进行RPR、TP-ELISA和TPPA检测。结果:RPR与TP-ELISA检测结果比较有显著性差异,P<0.01。在本组资料中,RPR真阳性15例(0.43%),TP-ELISA真阳性118例(3.39%),TPPA真阳性118例(3.39%)。TP-ELISA试验共有6例假阳性,占0.17%,假阴性无,而RPR试验共有12例假阳性,占0.34%,而假阴性103例,占2.96%。在3479例产妇中,RPR的总符合率为96.69%,灵敏度为12.71%,特异度为99.64%;TP-ELISA的总符合率为99.83%,灵敏度为100.00%,特异度为99.82%。结论:TP-ELISA敏感性高于RPR,因此可用高质量的TP-ELISA和RPR试剂对产妇进行筛查,应用TPPA试剂确证,可进一步保证医疗质量。TP-ELISA有高的敏感性和特异性,是产妇梅毒检测中的一个好的筛查方法。
Objective：To evaluate the rapid plasma reagin agglutination test （RPR） and Treponema pallidum en- zyme - linked immunosorbent assay （TP - ELISA） for the detection of pregnant syphilis in a clinical setting. Meth- ods ： Plasma and serum samples from 3479 pregnant women were tested for the presence of Treponema pallidum anti- gens by RPR and TP - ELISA and TPPA. Results： A statistical significant （P 〈 0.01 ） difference in test results were found between the RPR and TP- ELISA method. When using the RPR test, 15 cases （0.43%） were found positive, while 118 cases （3.39%） were found positive with the TP - ELISA and TPPA test. When the RPR test was used, there were 12 （0.34%） false positive samples and 103 false negative samples （2.96%） , respectively, while 6 （0.17%） false positive samples and no false negative samples were found with TP - ELISA. The total coin- cidence rate of the RPR test was 96.69%, and the sensitivity was 12.71% and the specificity was 99.64% ; The total coincidence rate of the TP - ELISA test was 99.83% , and the sensitivity was 100.00% and the specificity was 99.82%. Conclusion ： The TP - ELISA was more sensitive than the RPR test. TPPA test should be made to ensure the quality of the test results for puerpera syphilis. Study found that TP - ELISA has a high sensitivity and specifici- ty, can be a good test for pregnant women.
出处
《中国卫生检验杂志》
北大核心
2013年第2期397-398,共2页
Chinese Journal of Health Laboratory Technology
