摘要
目的:探索用微量铁盐培养基分离空肠弯曲菌,寻求分离空肠弯曲菌良好的培养基。方法:采用五种不同培养基,在相同条件下对空肠弯曲菌分离培养,观察其生长效果,用菌落计数法进行比较试验。结果:通过比较,发现含微量铁盐培养基对空肠弯曲菌分离效果好,检出率高。但也有遗漏,为了提高检出率,应与布氏菌血琼脂同时使用。结论:含微量铁盐培养基可用于空肠弯曲菌病流行病学调查和临床检验,空肠弯曲菌在该培养基上生长较快,菌落大,数量多,且制备简易、价廉,建议各实验室推广应用。
Objective ：To explore the effect of trace ferric salt medium in separation of Campylobacter jejuni, and find the optimal culture medium for Campylobacter jejuni. Methods ： The experiment was carried out under the same condition, Campylobacter jejuni were isolated and cultured with five different culture mediums. The growth of the bacteria was observed, the comparative experiment research was performed by colony counting method. Results： Af- ter comparing these five mediums, it was found the detection rate of trace ferric salt medium was remarkably higher than other culture medium in separation of Campylobacter jejuni, but there was also omission, in order to improve the detection rate, Campylobacter jejuni should be based on the simultaneous usage of the brucella blood AGAR and trace ferric salt medium. Conclusion： Trace ferric salt medium can be used for the disease epidemiological survey and clinical inspection of Campylobacter jejuni. The Campylobacter jejuni grows rapidly with large bacterial colony in large quantities on ferric salt medium, which is suggested to apply in laboratories for its low cost and easy prepara- tion.
出处
《中国卫生检验杂志》
北大核心
2013年第2期375-376,379,共3页
Chinese Journal of Health Laboratory Technology
关键词
微量铁盐培养基
空肠弯曲菌
Trace ferric salt medium
Campylobacter jejuni
