摘要
目的:建立蔬菜中49种农药残留量的分散固相萃取-气相色谱-串联质谱(QuEChERS-GC-MS/MS)快速分析方法。方法:样品用乙酸乙酯匀浆提取后,经分散固相萃取法净化,采用气相色谱-串联质谱法用选择反应监测模式SRM进行测定,内标法定量。结果:在0.01 mg/L～10.0 mg/L范围内各农药线性相关系数均大于0.99,分别在菜心、青瓜和土豆中添加浓度水平为0.01 mg/kg和0.02 mg/kg的混标,平均回收率为70.8%～122.5%,方法的相对标准偏差为0.7%～7.8%(n=6)。所有农药的方法定量限均小于10μg/kg。结论:该法前处理简单快速、灵敏、准确,具有良好的回收率和稳定性,较好的解决了样品本底成分复杂的基质干扰,适用于蔬菜中多种农药残留的测定。
Objective： A quick method was developed for the determination of 49 pesticide residues in vegetables by gas chromatography with tandem mass spectrometry （ GC - MS/MS） after dispersive solid - phase extraction. Methods： The target compounds were extracted from the samples with ethyl acetate and the extracted solution was cleaned up using dispersive solid - phase extraction. The collected solution was analyzed by GC - MS/MS. The tar- get pesticides were confirmed by using timed SRM with two transitions of each targets and quantified by internal standard method. Results： In the linear range （0.01 mg/L -10.0 mg/L） of each pesticide, the correlation coeffi- cient was higher than 0.99. The average recoveries （spiked at the levels of 0.01 mg/kg,0.02 mg/kg） were from 70.8 % - 122.5 %. The relative standard deviations were from 0.7% - 7.8 % （ n = 6）, and all limits of quantifica- tion（LOQs） were less than 10 μg/kg. Conclusion： The method is quick, sensitive, accurate, reproducible and suitable for the analysis of pesticide residues in vegetables.
出处
《中国卫生检验杂志》
北大核心
2013年第2期272-277,共6页
Chinese Journal of Health Laboratory Technology
基金
广东检验检疫局科技计划(2009GDK28)
中山市科技计划-农业科技攻关计划(20103A190)
关键词
分散固相萃取
气相色谱-串联质谱
农药残留
蔬菜
Dispersive - solid phase extraction
Gas chromatography - tandem mass spectrometry （ GC - MS/MS）
Pesticide residues
Vegetables
