摘要
目的:建立测定产复欣泡腾片中芍药苷含量的HPLC法。方法采用VP-ODS C18(4.6 mm×150 mm,5μm)色谱柱;流动相为乙腈-水-醋酸(75:425:0.4)为流动;检测波长为230nm。结果芍药苷在12.52～100.16μg/ml范围内线性关系良好(r=0.9998),平均回收率为99.54%,RSD=0.69%。结论本法简便、准确、重复性好,适用于产复欣泡腾片中芍药苷的含量测定。
Aim To establish a HPLC method for determination of Paeoniflorin content in Chanfuxin Effervescent Tablets. Methods ：VP -ODS C18 column was used. The mobile phase was acetonitrile - H20 - acetic acid （75：425 ：0. 4 ）, Detection wavelength was at 230nm. Results ： With in the range from 12.52 to 100.16μg . ml-1, Paeoniflorin presented a good relationship between the injected amounts and peak areas（ r = 0. 9998 ）. The average recovery was 99.54% and RSD was 0. 69%. Conclusion ： The method is simple, accurate, repeatable and can be ap- plied to determine Paeoniflorin content in Chanfuxin Effervescent Tablets
出处
《内蒙古中医药》
2013年第3期146-147,共2页
Inner Mongolia Journal of Traditional Chinese Medicine
