摘要
为探讨在盐胁迫下决明种子和幼苗各项生理生化指标的影响,采用NaCl模拟不同程度的盐胁迫,对盐胁迫下决明种子的发芽率、发芽指数、活力指数、胚根长、下胚轴长度及鲜质量、幼苗叶片MDA和O2-.质量分数及抗氧化酶活性指标的生理特性进行了测定,结果表明:当盐浓度为50mmol/L及其以上时,随着胁迫浓度和胁迫时间的增加决明种子的发芽率、发芽指数、活力指数均显著降低;决明幼苗胚根长、下胚轴长和鲜质量也表现出缩短和下降现象;幼苗叶片中丙二群(MDA)和超氧阴离子(O2-.)质量分数呈上升趋势;超氧化物歧化酶(SOD)活性呈先上升后下降的趋势,而过氧化氢酶(CAT)和过氧化物酶(POD)活性则在胁迫初期随着胁迫浓度的增加呈上升的趋势,在胁迫的中后期随着胁迫浓度和时间的增加呈先升后降的趋势.盐胁迫对决明种子萌发和幼苗生长具有显著的抑制作用,但决明种子及幼苗通过自身的调节也主动适应盐胁迫环境条件.
In order to explore the effects of NaC1 stress on seeds germination and seedling growth of Cassia obtusifolia L. and on its physiological characteristics indexes, NaC1 stress, created with different concen- trations of NaC1, have been studied in this research, and its physiological characteristics indexes including germination rate, germination index, vigor index, radical length, hypocotyl length, fresh weight, the MDA content, O2^- content, and antioxidant enzyme activity have also been measured. The results showed that,the effects of low concentrations under NaC1 stress on its physiological characteristics of Cassia ob-tusifolia L. a is not obvious, but with the strengthening of NaC1 stress（50 mmol/L） and extending of the stress time , seeds germination rate, germination index, vigor index , fresh weight were significantly re-duced, radical length, hypocotyl length were shortened. The MDA content and O2^- content show an up- ward trend, SOD activity increases first and then decreases, and in the Preliminary stage CAT and POD ac-tivity have been increased, but with the strengthening and time of NaC1 stress CAT and POD activity have first increased and then decreased in the metaphase and later stage. The seeds germination and seeding growth of Cassia obtusifolia L. have obviously inhibited by NaC1 stress, but the plant could adapt to NaC1 stress through its own changes.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第2期73-78,共6页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家林业公益科研专项资助项目(201004064)
国家自然科学基金资助项目(30070080)
关键词
决明
盐胁迫
种子萌发
幼苗生长
生理生化指标
Cassia obtusifolia
NaC1 stress
seeds germination
seeding growth
physiological and bio-chemical indexes
