摘要
目的探讨高迁移率蛋白A2（HMGA2）和Smad4在胃腺癌组织中差异性表达的临床意义及其与上皮间质转化的关系。方法通过免疫组织化学方法分别检测80例胃腺癌癌组织和20例胃阴性切端中HMGA2、Smad4、E—cadherin和vimentin的蛋白表达水平，并对其与病理分级和临床分期之间的相关性作统计学分析。结果HMGA2和vimentin在胃腺癌中的蛋白阳性表达率分别为41．25％（55／80）和67．5％（54／80），表达水平分别高于胃阴性切端组织（P〈0．05）。Smad4和E—cad在胃腺癌中的阳性表达率分别为17．5％（14／80）和26．25％（21／80），表达水平分别低于胃阴性切端组织（P〈0．05）；HM—GA2与vimentin呈正相关（P〈0．05），与Smad4、E—cadherin呈负相关（P〈0．05）。HMGA2与胃腺癌患者的分化程度、TNM分期和淋巴结转移有显著的相关性，Smad4与胃腺癌患者的浸润深度、TNM分期和淋巴结转移有显著的相关性。结论HMGA2、vimentin的表达上调和E—cadherin、Smad4的表达下调可以促进上皮间质转化（EMT），与胃腺癌的发生、发展和转移密切相关，HMGA2和Smad4可能作为胃癌诊断和预后的生物学标记。
Objective To investigate differential expression of HMGA2 and Smad4 in gastric carcinoma and its significance. To investigate association between HMGA2 and epithelial mesenehymal transitions. Methods Tissue samples from 80 gastric carcinoma tissues and 20 adjacent tissues were examined by immunohistochemistry for the expressions of HMGA2, Smad4, E - cadherin and vimentin. Results The positive expressions of HM- GA2 and vimentin were 41.25% （55/80）and 67. 5% （54/80）, respectively. It is higher than that of adjacent tissues（ P 〈 0.05 ）. The positive expression of Smad4 and E - cad were 17.5% （14/80） and 26.25% （ 21/80 ） respectively, which is lower than that of adjacent tissues （P 〈 0.05 ）. HMGA2 and vimentin expression in the gastric carcinoma was positive correlation; Smad4, E - cadherin and HMGA2 in the gastric carcinoma was negative correlation. The expression of HMGA2 was not related to the age and sexual, but it was correlated with differentiation, TNM stage and lymph node metastasis （P 〈 0.05 ）. The expression of Smad4 was correlated with infiltration, TNM stage and lymph node metastasis（ P 〈 0. 05 ）. Conclusion The upregulated expression of HMGA2 ,vimentin and the down regulated expressions of Smad4 and E - cadherin will promote the growth and the lymph node metastasis of gastric cancinoma in some degree. HMGA2 and Smad4 can be regarded as very important biologic markers in the gastric cancer diagnosis and prognosis.
出处
《实用肿瘤学杂志》
CAS
2013年第1期26-30,共5页
Practical Oncology Journal
基金
黑龙江省自然基金（D200921）
关键词
胃腺癌
高迁移率蛋白A2
SMAD4
上皮间质转化
Gastric carcinoma
High mobility group protein A2 （HMGA2）
Smad4
Epithelial mesenchy-real transitions（EMT）
