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猪孕酮受体基因的遗传变异及其与繁殖性状的相关性 被引量：1

Genetic variation of progesterone receptor gene and its association with reproductive traits in pig
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摘要 以猪孕酮受体(PGR)基因序列为模板设计引物,采用PCR RFLP技术检测PGR基因外显子1在长白猪和大白猪中的单核苷酸多态性,同时研究该基因对猪总产仔数、产活仔数和出生窝重的影响。对于扩增的外显子1片段,在大白猪中检测到GG、AG和AA型,在长白猪中只检测到GG和AG型。测序结果表明,GG型与AA型相比,在326 bp处有一单碱基突变(G→A),该基因所编码的氨基酸则由甘氨酸变为丝氨酸。长白猪GG型总产仔数、产活仔数和出生窝重与AG型总产仔数、产活仔数和出生窝重的差异均没有统计学意义。大白猪AG型总产仔数、产活仔数和出生窝重与GG型总产仔数、产活仔数和出生窝重的差异有统计学意义,大白猪GG型总产仔数、产活仔数和出生窝重与AA型总产仔数、产活仔数和出生窝重的差异没有统计学意义,大白猪AG型总产仔数、产活仔数与AA型总产仔数、产活仔数的差异有统计学意义,大白猪AG型出生窝重与AA型出生窝重的差异没有统计学意义。 PCR-RFLP were developed using the primers designed based on the sequence of PGR gene to determine the single-nucleotide polymorphisms （SNPs） of exon 1 of PGR gene in Landrace and Large White sows. And the influence of PGR gene on the total number born （TNB）, number born alive （NBA） and litter birth weight （LBW） was also investigated. There were 3 genotypes （GG, AG and AA） in Large White sows, and two genotypes （GG and AG） in Landrace sows. There was a single base mutation （G→,A） between GG genotype and AA genotype at position 326 bp, which caused a change of amino acid residue （Glycine→Serine）. In Landraee sows, the differences of TNB, NBA and LBW between GG genotype and AG genotype did not approach statistical significance （P〉0.05）. In the Large White sows, the differences of TNB, NBA and LBW between AG genotype and GG genotype all reached statistical significance （P〈0.05）, while those differences between GG genotype and AA genotype did not reach statistical significance （P〉0.05）. The difference of TNB and NBA between AG genotype and AA genotype of Large White sows reached statistical significance （P〈0.05）, but the difference of LBW did not reach statistical significance （P〉0.05）.
作者 张善文 陈斌
机构地区 湖南农业大学动物科学技术学院
出处 《湖南农业大学学报（自然科学版）》 CAS CSCD 北大核心 2013年第1期58-62,F0003,共6页 Journal of Hunan Agricultural University(Natural Sciences)
基金 国家现代农业产业技术体系建设专项(CARS 36)
关键词 猪 孕酮受体 遗传变异 繁殖性状 pig PGR gene genetic variation reproductive traits
分类号 S828 [农业科学—畜牧学]
  • 相关文献

参考文献12

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共引文献29

同被引文献15

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湖南农业大学学报（自然科学版）
2013年 第1期

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