摘要
目的 探讨胆囊胆固醇结晶形成与载脂蛋白E(ApoE)的关系。方法 应用聚合酶链反应 (PCR)技术检测 80例胆囊胆固醇结石患者的ApoE基因多态性 ;采用偏振光显微镜观察 80例胆囊胆固醇结石患者胆汁中胆固醇单水结晶的形成及数目。结果 80例胆囊胆固醇结石患者中有E4型 2 3例 ,其中 15例术后立即观察到胆固醇单水结晶 ,而E3 型 49例中有 2 0例术后立即观察到结晶 ,E2 型 8例中有 3例立即观察到结晶 ,E4 型明显高于E3 及E2 型 (P <0 .0 1) ,且ApoE4 型胆固醇单水结晶数目 (130 0 /mm3)明显高于E3(5 2 0 /mm3)及E2 (5 0 0 /mm3)。E4 型成核时间 (2 .5d)比E3(5 .5d)和E2 (6 .0d)明显缩短 (P <0 .0 1)。结论 胆囊胆固醇结晶形成与ApoE相关 ,ApoE4 为胆囊胆固醇结晶形成的一种遗传易感性因子。
Objective\ To study the relationship between cholesterol crystalization in gallbladder and apoliprotein E (ApoE). Methods\ The polymerase chain reaction (PCR) technique was used to detect the polymorphism of ApoE gene in 80 cases of cholesterol gallstones;polarized light microscope to detect the number and development of cholesterol monohydrate crystals in 80 cases of cholesterd gallstones. Results\ Of 80 patients with cholesterol gallstones,E 4 was detected in 23.And on 15 of 23,cholesterol monohydrate crystals were detected immediately after surgery.In 20 of 49 E 3 patinents,cholesterol crystal was detected immediately after surgery. In 3 of 8 E 2 cases,cholesterol crystal was also detected immediately after surgery.E 4 is significantly higher than E 3 and E 2 ( P <0.01). And the number of cholesterol monohydrate crystal (1?300/mm 3) is significantly higher than that of E 3 (520/mm 3) and E 2 (500/mm 3). The nuclei time of E 4 (2.5 days) is significantly shorter than that of E 3 (5.5 days) and E 2 (6.0 days) ( P <0.01). Conclusion\ The development of cholesterol gallstone is closely related with ApoE gene polymorphism.And the ApoE 4 is a genetic factor in the development of cholesterol gallstone.\;
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第6期520-520,共1页
Chinese Journal of Experimental Surgery
基金
辽宁省自然科学基金!资助项目 (972 2 77)
