摘要
目的:探讨肥胖对小鼠肺部炎症及肺功能的影响。方法:建立肥胖小鼠模型,雄性ICR小鼠母乳喂养21 d离乳,随机分为高脂组与正常组,分别给予高脂与标准饲料喂养150 d。将高脂组中超过正常体质量的10%～20%定义为超重组,超过正常体质量的20%定为肥胖组。进行基础肺功能测定,肺组织HE染色、巨噬细胞特异性标记抗原F4/80免疫组化。结果:(1)肺组织HE染色和免疫组化F4/80示肥胖组支气管周围、肺泡间隔炎性细胞浸润较正常组、超重组明显增多,以巨噬细胞增多为主。(2)肺功能测定显示肥胖组最大吸气流速、最大呼气流速、呼出50%潮气量时呼气流速相对于正常组和超重组无统计学差异(P>0.05)。结论:肥胖可促进肺部炎性细胞浸润,但对通气功能影响较小。
Objective：To detect the effects of obesity on pulmonary inflammation and function in mice. Methods： Male ICR mice were fed with high fat diet for 150 d from the 21st d postnatal after weaning to establish obese mice model（high fat group） while those in control group were given standard chow diets. Mice in high fat group with weight 10%-20% heavier than the normal were classified into overweight group;mice in high fat group with weight 20% heavier than the normal were classified into obesity group. Basic lung function was recorded;pathological HE staining and immunohistochmistry labeling maerophages （F4/80） were performed to measure lung inflammation. Results：（ 1 ）Pathological HE staining and immunohistochmistry （F4/80） of pulmonary tissues showed that inflammatory cell infiltration in alveolar septum and around bronchus was increased in obesity group than in control group and overweight group（especially macrophages ）. （2） Pulmonary function （ peak inspiratory flow ; peak expiratory flow ; 50% tidal volume expiratory flow） showed no significant difference between these groups. Conclusions：Obesity enhances lung inflammation, however, makes no difference in ventilation function.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2013年第2期113-116,共4页
Journal of Chongqing Medical University
基金
国家"十一.五"科技支撑计划基金资助项目(编号:2007BA124B05)
关键词
肥胖
肺
炎症
肺功能
obesity
lung
inflammation
pulmonary function
