摘要
目的 了解恶性疟原虫云南株 PFD- 3/ YN pfs2 5基因序列变异情况。方法 PCR扩增恶性疟原虫云南株 PFD- 3/YN株 pfs2 5全基因 ,经 Sanger双脱氧链终止法测序和 Pst I酶切鉴定 ,并与国外恶性疟原虫 NF4株 pfs2 5进行比较。结果 恶性疟原虫云南株 PFD- 3/ YN株 pfs2 5基因与 NF4株的 pfs2 5基因其同源性为 99.2 %。结论 PFD- 3/ YN株与 NF4株 pfs2
Malaria vaccines are being developed against different stages in the parasite's life cycle.Although not directly protective,the sexual stage vaccines would induce antibodies that would prevent infection of mosquitoes when ingested in a bloodmeal containing sexual stage parasites.pfs25 has been tested to be an important candidate antigen for malarial transmission blocking vaccine .In this report we analyzed the complete code of pfs25 gene of Plasmodium falciparum PFD-3/YN isolate.The result shows that the gene encoding pfs25 of PED-3/YN isolate has a mutant which generates a PstI endonuclease restriction site and shares 99.2% nucleotide homology with that of NF4 isolate. \ \
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第6期19-20,共2页
Chinese Journal of Zoonoses
基金
国家自然科学基金!资助 (编号39700124)* Genebank编号AF193769
