摘要
目的 :探讨BMPs家族成员与外周神经肿瘤的关系。方法 :用原位杂交方法对BMP 2 ,3,4,5在人良、恶性神经鞘瘤中的表达进行观察。结果 :显示BMP 2 ,3,4,5mRNA在外周神经的良恶性神经鞘瘤中均有分布。良性肿瘤中 ,呈栅栏状排列 ,肿瘤细胞聚集的区域BMPs表达升高 ;恶性肿瘤中BMPs的表达高于良性肿瘤。结论 :确定了BMPs在外周神经肿瘤中的表达和分布 ,揭示部分BMPs可能参与了外周神经肿瘤的发生发展过程。
Objective: The aim of present study is to elucidate the relationship between the members of BMP family and the neoplasms of peripheral nerve. Methods: Eight cases of schwannoma and three cases of malignant schwannoma were collected and all samples were fixed with 10% neutral formalin and embedded in paraffin wax. Five μm thickness sections were prepared for staining with haematoxylin and eosin for histopathological assessment and processed for in situ hybridization for assessing the mRNA transcriptions of BMP 2,3,4,5. Sections of osteosarcoma that previously demonstrated consistent expression of BMP 2 mRNA were included in each batch of staining to serve as positive control. Results: The results showed that all of the neoplastic lesions of nerve sheath showed a consistent expression of BMP 2,3,4,5. The positive signals of benign lesions were obviously increased in the palisading area or the area of accumulated cells. There was no detectable staining in the matrix of tumour and collagenous fiber. The staining intensity was relatively and slightly weak in cells of Antoni B tissue when compared with Antoni A tissue. Nevertheless, the tumour cells in Antoni B tissue also showed BMP 2,3,4 and 5 positively staining. The fibroblasts of schwannoma were negatively stained. The malignant lesions showed higher expression signals of BMPs than benign lesions. Conclusion: The results can be concluded that the selected members of BMPs may be involved in the process of growth and progression of the neoplasms of peripheral nerve. \;
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2000年第5期307-309,共3页
West China Journal of Stomatology
关键词
BMP
原位杂交
颌面部神经鞘瘤
BMP\ \ nerve sheath tumor\ \ malignant\ \ in situ hybridization
