摘要
通过形态学观察、诱导产孢、致病力测定和分子生物学研究,对引起辽宁地区越橘枝枯病的病原菌进行鉴定,确定其为葡萄座腔菌(Botryosphaeria dothidea)。病原菌在越橘植株上接种后表现枝条枯萎、木质部变色等症状,与田间自然发病植株一致;在PDA培养基上菌落初期为白色,后转为灰黑色,分生孢子纺锤形或梭形,无色,单胞,内含多个油滴;水琼脂+麦芽提取物、苹果枝条和苹果幼果3种病原菌培养方法均可诱导产孢,苹果幼果是诱导病原菌产孢的最佳基质。供试菌株LB1的rDNA-ITS和β-tubulin基因序列与GenBank中已有的Botryosphaeria dothidea序列相似性分别达到99%与100%。
The pathogen causing stem blight on blueberry in Liaoning, China was identified as Botryosphaeria dothidea by morphological characteristics, sporulation features, pathogenicity test, and the molecular identification. The stems showed symptoms of dieback, discoloration in thinks and death after inoculating with the pathogen, which were consistent with the symptoms of the natural infected plants. All isolates showed the colonies white at the early stage, then became gray to black, conidia were hyaline, aseptate, fusiform with several oil globules, young apple fruit is the suitable medium for sporulation. Identity of isolate LB 1 was confirmed by analysis of the rDNA-ITS and β-tubulin sequences with primers ITS1 -ITS4 and Bt2a- Bt2b. BLAST searches showed 99% and 100% identity with Botryosphaeria dothidea isolates from GenBank.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第2期231-236,共6页
Acta Horticulturae Sinica
基金
中国农业科学院基本科研业务费预算增量项目(2013ZL019)
公益性行业(农业)科研专项(201103037)
