摘要
以‘藤稔’葡萄为试材,利用半定量RT-PCR和荧光定量PCR技术对5个葡萄氮代谢基因VvGHD(JF796045)、VvNiR(JF796046)、VvNR(JF796047)、VvGS(JF796048)和VvAS(JF796049)在施用尿素后的表达情况进行了分析。5个基因在葡萄叶面喷施尿素后不同时间段的表达量存在显著差异,整体呈先升高后降低的趋势;VvGDH最高表达水平发生在尿素喷施后48h,VvNR和VvNiR最高表达水平发生在24h,VvGS最高表达水平发生在6h,VvAS最高表达水平发生在2h。叶面喷施比土壤施用尿素叶中5个基因表达高峰出现的时间早,且表达水平高;土壤施尿素后叶中5个基因对尿素的响应时间比根中延后,但响应强度明显高于根中。
To understand gene response to fertilizer application, quantitative l＇uK ann semi- quantitative PCR were used to analyze the expression of five genes （ VvGHD, VvNiR, VvNR, VvGS and VvAS） of‘Fujiminori＇ grape at different times with the fertilizer application. The results show that the expression levels of five genes exhibit significant differences at different times after treatment, the overall trend was first increased and then decreased; The highest expression level of VvGDHwas in 48 h, VvNR and VvNiR the highest expression level in 24 h, VvGSthe highest expression level in 6 h, VvASthe highest expression level in 2 h. The highest expression levels of five genes after ground fertilization in leaves were delayed longer than those after foliar fertilizer application, and the overall expression level lower than foliar fertilizer application; Response of five genes after ground fertilization in leaves was delayed longer than the root, but the response intensity was significantly higher than corresponding genes in the root.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第2期221-230,共10页
Acta Horticulturae Sinica
基金
江苏省农业综合开发科技推广项目(2011KJ-53)
教育部新世纪优秀人才支持计划项目(NCET-08-0796)
关键词
葡萄
氮代谢
基因
表达
grapevine
nitrogen metabolism
gene clone
gene expression
