摘要
目的 :探讨p5 3基因单链构象多态性变化与食管鳞癌患者术后生存期的关系。方法 :采用聚合酶链反应 单链构象多态性 (PCR SSCP)结合银染技术 ,对 47例河南林县食管鳞癌患者癌组织及癌旁正常组织中p5 3基因第 5～ 8外显子进行比较。结果 :p5 3基因第 5～ 8外显子出现异常电泳条带者 2 0例 (4 2 6 % ) ;生存期大于 5a组与生存期小于 5a组 ,p5 3基因单链构象多态性变化发生率分别为 32 1% (9/ 2 8)和 5 7 9(11/ 19) ,2组比较 ,差异无显著性(χ2 =3 0 71,P >0 0 5 )。结论 :食管鳞癌组织p5 3基因第 5～
Aim: To investigate the relationship between the single strain conformation polymorphism alteration of p53 and the survival rate in the patients with esophageal squamous cell carcinoma Methods: PCR SSCP methods and silver staining technique were used to analyse the alteration of p53 gene in the exons 5～8 in 47 cases with esophageal squamous cell carcinoma Results: Abnormal shift bands in the exons 5～8 of the p53 gene were found in 20 cases out of 47 patients with esophageal squamous cell carcinoma,which accounted for 42 6%(20/47) The alterations of p53 gene were found more frequently in the patients with less than 5 years survival rate (57 9%,11/19) than those in the patients with longer than 5 years survival rate (32 1%,9/28). However,the difference was not significant ( P >0 05) Conclusion: The result suggests that p53 gene single strain conformation polymorphism alteration may not play an important role in the prognosis for the patients with esophageal squamous cell carcinoma
出处
《河南医科大学学报》
2000年第6期512-514,共3页
Journal of Henan Medical University
基金
河南省自然科学基金资助! 95 40 2 0 90 0
