摘要
目的建立混凝沉淀富集回收水中隐孢子虫卵囊的方法。方法以4μm荧光微球为研究对象,采用混凝沉淀试验,通过正交设计筛选出最佳混凝剂[聚合氯化铝(PAC)、水溶性壳聚糖(WSC)、1 mol/L的NaOH溶液用量]的配方,并观察不同pH值(6～9)、浑浊度(0～15 NTU)和水温(5～15℃)对回收率的影响。结果确定了最佳混凝配方,聚合氯化铝浓度为80 mg/L,壳聚糖浓度为30 mg/L,1 mol/L氢氧化钠溶液用量为0.6 ml。当pH值在6～9范围内,回收率均在86%以上;当水温在5～25℃范围内,回收率在55%以上;当浑浊度为0～15 NTU范围内,回收率均在90%以上。且回收率均随着pH值、水温和浑浊度的升高而上升。向实际水样投加隐孢子虫,采用混凝沉淀法与EPA1623法进行富集比较,差异无统计学意义。结论该方法能够高效富集回收水中的隐孢子虫卵囊,并对实验条件要求较低,适用于一般实验室对水中隐孢子虫卵囊的检测。
Objective To establish a method for coagulating sedimentation to enrich and recover Cryptosporidium oocysts from surface water,and to provide theoretical guidance for the C. oocysts correlational research in water. Methods 4 μm fluorescent microspheres as the research object,the optimum coagulation formulation was screened using the jar test by orthogonal design, and the effects of influencing factors such as pH （6-9）, turbidity （0-15 NTU）, and temperature （5-15 ℃ ） on recovery were observed. Results The optimum coagulation formula was 80 mg/L PAC, 30 mg/L WSC and 1 mol/L NaOH 0.6 ml. Recovery rates of fluorescent microsphere were all above 86% in pH range of 6-9; At temperature of 5-25 ℃ ,recovery rates were all over 55% ;The turbidity of the range of 0-15 NTU,the recovery rates were all over 90%. And as pH,temperature and turbidity increased,the recovery rate also increased. The recoveries of Cryptosporidium oocyst in actual water samples were determined by using coagulating sedimentation and national standard method respectively, no significant difference was seen between the two methods. Conclusion Coagulating sedimentation method can efficiently reclaim Cryptosporidium oocysts in water, with low cost in replacing the disposable filter capsules and low requirements in experimental conditions, it is applicable to the determination of Cryptosporidium oocyst in water for a common laboratory.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2013年第2期102-104,共3页
Journal of Environment and Health
基金
国家高技术研究发展计划(863计划)项目(2008AA062502-2)
关键词
混凝
沉淀
荧光微球
隐孢子虫卵囊
Coagulation
Sedimentation
Fluorescent microspheres
Cryptosporidium oocyst
