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CRISPR结构与功能研究进展 被引量：9

Advances in research on structure and function of CRISPR
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摘要 研究发现,在细菌纲和古细菌纲中发现的规律成簇间隔短回文重复序列(clustered regularly interspacedshort palindromic repeat,CRISPR)构建了一种特殊的防御系统,能够有效地抵抗噬菌体和外界各种基因元件对其造成的干扰。CRISPR因其在结构上的特殊性以及功能上的特异性正逐渐成为细菌研究领域的热点。本文综述了近年来有关CRISPR系统的作用机制、分类、结构与相关功能的研究进展。 Clustered regularly interspaced short palindromic repeats （CRISPR） found in Archaea and Bacteria encode an adaptive immune system, which provides effective resistance against phages and foreign nucleic-acid elements. CRISPR is becoming a hot spot in the field of bacteriology because of the unusual structure and specific function. The research progress in the structure, classification, function, and mechanism of the CRISPR system is reviewed.
作者 杨超杰 邱少富 宋宏彬
机构地区 军事医学科学院疾病预防控制所
出处 《军事医学》 CAS CSCD 北大核心 2013年第2期155-158,共4页 Military Medical Sciences
基金 国家科技重大传染病专项资助项目(2013ZX10004607 2012ZX10004215 2013ZX10004218)
关键词 规律成簇间隔短回文重复序列 作用机制 结构 功能 CRISPR mechanism structure function
分类号 R341 [医药卫生—基础医学] Q933 [生物学—微生物学]
  • 相关文献

参考文献32

  • 1Bikard D, Hatoum-Aslan A, Mucida D, et al. CRISPR interference can prevent natural transformation and virulence acquisition during in vivo bacterial infection[J]. Cell Host Microb ,2012,12 (2) : 177 -186.
  • 2崔玉军,李艳君,颜焱锋,杨瑞馥.规律成簇的间隔短回文重复：结构，功能与应用[J].微生物学报,2008,48(11):1549-1555. 被引量：11
  • 3Deveau H, Garneau JE, Moineau S. CRISPR/Cas system and its role in phage-bacteria interactions [ J ]. Ann Rev Microbiol, 2010,64 : 475 -493.
  • 4Barrangou R, Fremaux C, Deveau H, et al. CRISPR provides acquired resistance against viruses in prokaryotes [ J ]. Science, 2007,315(5819) : 1709 - 1712.
  • 5Brouns SJJ, Jore MM, Lundgren M, et al. Small CRISPR RNAs guide antiviral defense in prokaryotes [J]. Science, 2008,321 (5891) : 960 -964.
  • 6aGarneau JE, Dupuis MS, Villion M, et al. The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA [J]. Nature ,2010,468 (7320) : 67 - 71.
  • 7Makarova KS, Haft DH, Barrangou R,et al. Evolution and classi-fication of the CRISPR-Cas systems [ J ]. Nat Rev Micmbiol, 2011,9(6) : 467 -477.
  • 8Marraffini LA, Sontheimer EJ. CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea [ J ]. Nat Rev Genet,2010,11 (3) : 181 - 190.
  • 9Sinkunas T, Gasiunas G Fremaux C,et al. Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/ Cas immune system[J]. EMBO J,2011,30(7) : 1335 -1342.
  • 10Deltcheva E, Chylinski K, Sharma CM, et al. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase Ⅲ [J]. Nature,2011,471 (7340) : 602 -607.

二级参考文献39

  • 1Sturino JM, Klaenhammer TR. Engineered bacteriophage-defenc e systems in bioprocessing. Nat Rev Microbiol, 2006, 4(5): 395-404.
  • 2Jansen R, Embden JD, Gaastra W, et al. Identification of genes that are associated with DNA repeats in prokaryotes. Mol Microbiol, 2002, 43 (6): 1565-1575.
  • 3Barrangou R, Fremaux C, Deveau H, et al. CRISPR provides acquired resistance against viruses in prokaryotes. Science, 2007, 315 (5819): 1709-1712.
  • 4Sorek R, Kunin V, Hugenholtz P. CRISPR - a widespread system that provides acquired resistance against phages in bacteria and archaea. Nat Rev Microbiol, 2008, 6(3): 181-1816.
  • 5Grissa I, Bouchon P, Pourcel C, et al. On-line resources for bacterial micro-evolution studies using MLVA or CRISPR typing.Biochimie, 2008, 90(4): 660-668.
  • 6Grissa I, Vergnaud G, Pourcel C. The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats. BMC Bioinformatics, 2007, 8(1): 172.
  • 7Mojica FJ, Diez-Villasenor C, Sofia E, et al. Biological significance of a family of regularly spaced repeats in the genomes of Archaea, Bacteria and mitochondria. Mol Microbiol, 2000, 36(1): 244-246.
  • 8Makarova KS, Grishin NV, Shabalina SA, et al. A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Biol Direct, 2006, 3: 1-7.
  • 9Ishino Y, Shinagawa H, Makino K, et al. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol, 1987, 169 (12): 5429-5433.
  • 10She Q, Singh RK, Confalonieri F, et al. The complete genome of the crenarchaeon Sulfolobus solfataricus P2. Proc Natl Acad Sci USA, 2001, 98 (14): 7835-7840.

共引文献10

同被引文献60

  • 1赵宇亮.纳米颗粒的毒性问题[J].创新科技,2007(4):44-45. 被引量：4
  • 2Horvath P, Barrangou R. CRISPR/Cas, the immune system of bacteria and archaea[ J ]. Science,2010,327 (5962) : 167 - 170.
  • 3Sorek R, Kunin V, Hugenholtz P. CRISPR-a widespread system that provides acquired resistance against phages in bacteria and archaea[ J]. Nat Rev Microbiol,2008,6(3 ) : 181 - 186.
  • 4Iranzo J, Lobkovsky AE, Wolf YI, et al. Evolutionary dynamics of the prokaryotic adaptive immunity system CRISPR- Cas in an explicit ecological context[ J]. J Bacteriol,2013,195 (17) :3834 -3844.
  • 5Grissa I, Vergnaud G, Pourcel C. The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats [ J]. BMC Bioinformatics,2007,8 ( 1 ) :172 - 182.
  • 6MojicaFJM, Garcla-Marffnez J, Sofia E, et al. Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements[ J]. J Mol Evol,2005,60(2) :174-182.
  • 7Marraffini LA. CRISPR-Cas immunity against phages : its effects on the evolution and survival of bacterial pathogens [J]. PLoS Pathogens,2013,9 ( 12 ) : e103765.
  • 8Abedou ST. Facilitation of CRISPR adaptation [ J ]. Bacterophage ,2011,1 (3) : 179 - 181.
  • 9Van der Oost J, Jore MM, Westra ER, et al. CRISPR-based adaptive and heritable immunity in prokaryotes [ J]. Trends Biochem Sci,2009,34 (8) :401 - 407.
  • 10Sun CL,Barrangou R, Thomas BC, et al. Phage mutations in response to CRISPR diversification in a bacterial population [ J ]. Environ Microbiol.2013.15 ( 2 ) ,463 - 470.

引证文献9

二级引证文献37

军事医学
2013年 第2期

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