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肺炎克雷伯菌全基因组DNA芯片的研制和质量评价

Development and evaluation of whole-genome DNA microarray of Klebsiella pneumoniae
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摘要 目的研制肺炎克雷伯菌(KP)全基因组DNA芯片并对芯片质量进行评价。方法根据KP的NTUH-K2044全基因组序列,挑选出5075条基因,PCR扩增各基因并纯化产物,点样制备芯片。设计了3个质控杂交组合,采用双色荧光杂交策略,对芯片质量进行评价。结果与结论芯片杂交结果与理论预期结果一致。本研究成功研制了KP全基因组DNA芯片,并建立了基于全基因组DNA芯片的KP比较基因组学技术平台。 Objective To develop and evaluate a whole-genome DNA mieroarray of Klebsiella pneumoniae. Methods Based on genomic sequences of K. pneumoniae NTUH-K2044, a total of 5075 genes were chosen, amplified by PCR and printed onto glass slides. Three sets of two-fluorescence comparative hybridizations were performed to evaluate the quality of the microarray. Results and Conclusion Microarray hybridization results were consistent with the anticipated results. The whole-genome DNA microarray of K. pneumoniae is successfully developed, and a method of microarray-based comparative genomic analysis of K. pneumoniae is established accordingly.
作者 刘梦颖 张义全 王丽 闫小娟 杨世亚 周冬生 杨瑞馥 邱景富
机构地区 重庆医科大学公共卫生与管理学院 军事医学科学院微生物流行病研究所
出处 《军事医学》 CAS CSCD 北大核心 2013年第2期122-125,共4页 Military Medical Sciences
基金 国家自然科学基金资助项目(31071093,31170129) 重庆市科委自然科学基金资助项目(2010BB5103)
关键词 克雷伯菌 肺炎 DNA芯片 比较基因组学 Klebsiella pneumoniae DNA microarray comparative genomics
分类号 R346 [医药卫生—基础医学]
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参考文献10

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军事医学
2013年 第2期

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