摘要
以林可霉素(LIN)为模板,利用分子模拟方法模拟丙烯酰胺(AM)、甲基丙烯酸(MAA)、2-乙烯基吡啶(2-VP)和4-乙烯基吡啶(4-VP)4种常见的功能单体与模板分子的结合能,结果表明4-VP结合能最强为-98.25 kJ/mol。用悬浮聚合法制备林可霉素分子印迹聚合物(MIP)并对其性能进行评价。使用气相色谱检测各功能单体制备的印迹聚合物的吸附量,结果4-VP制备的印迹聚合物吸附量最大为102.8μmol/g,分子模拟结果与吸附试验结果一致,表明分子模拟可以为功能单体选择提供依据。采用静态吸附试验和动态吸附试验对印迹聚合物吸附性能表征,并进行了Scatchard模型分析。结果印迹因子为2.54,并且在150 min内较快地达到吸附平衡,表明林可霉素分子印迹聚合物具有较高的选择吸附性,可用于林可霉素的富集。
The interaction binding energies between lincomycin （LIN） using as template of molecularly imprinted polymers （MIP） and function monomers are calculated by molecular simulation through Material Studio. Aerylamide （ AM ）, Methylacrylie acid （ MAA）, 2-Vinylpyridine （2-VP） and 4-Vinylpyridine （4-VP） are taking as function monomers. According to molecular mechanics eaculation, LIN with 4-VP has the highest binding energy which was -98.26 kJ/mol. Then MIP has been prepared using LIN with the above monomers respectively by suspension polymerization. The adsorption capacity of MIP was testing by gas chromatography （GC）. The results show that the complex of LIN with 4-VP has the maximum adsorption capacity, which was 102. 8 μmol/g. It means that the binding capacity of it is stronger than other function monomers which is the same to the analysis of molecular mechanics eaculation. 4-VP was selected as the best function monomer for MIP. High retention factor and selective adsorption ability were achieved by static adsorption experiment, dynamic equilibrium adsorption experiment and Scatchard analysis. Imprinted factor was 2. 54 and the equilibrium adsorption reached about 150 min. MIP was successfully applied to selective separation and preconcentration of LIN.
出处
《分析试验室》
CAS
CSCD
北大核心
2013年第3期26-29,共4页
Chinese Journal of Analysis Laboratory
基金
国家"十一五"科技支撑项目子项目(2006BAK02A08-4)
安徽高校省级自然科学研究项目(KJ2011B065)资助
关键词
分子印迹
分子模拟
林可霉素
悬浮聚合
气相色谱
Molecular imprinted polymer
Molecular simulation
Licomycin
Suspension polymerization
Gas chromatography
