摘要
以无血清的RPMI-1640培养液灌洗奶牛腹腔,分离获取奶牛腹腔巨噬细胞,在含10%小牛血清的RPMI-1640培养液中培养。采用倒置显微镜观察细胞形态,并通过瑞氏染色计算其纯度。结果表明,采用无血清的RPMI-1640培养液灌洗奶牛腹腔,能够获得较高纯度的巨噬细胞,能为奶牛胞内寄生菌存活机制的研究提供很好的试验材料。
The peritoneal cavity of dairy cow was douched with RPMI - 1640 culture medium without fe- tal calf serum （FCS）, and the peritoneal macrophages were separated and cultured in RPMI -1640 medium with 10% FCS. And then the cell morphology was observed under inverted microscopy, and the purity of mac- rophages was analyzed by Wright＇ s staining. The results showed that highly purified macrophages were ob- tained by this method, which could be prepared for researches on the mechanism of intracellular bacterium liv- ing in dairy cow.
出处
《山东农业科学》
2013年第2期38-40,共3页
Shandong Agricultural Sciences
基金
公益性行业(农业)科研专项(200903027)
现代农业(奶牛)产业技术体系岗位科学家专项
关键词
奶牛
巨噬细胞
分离培养
Dairy cow
Macrophage
Separation and culture
