摘要
目的用红外光谱方法分析氟对实验动物亚细胞结构的影响。方法将32只无特定病原体级纯种昆明小鼠随机分为低氟、中氟、高氟3个染氟组和对照组,染氟组分别给予腹腔注射氟化钠2、8、32 mg.(kg.d)-1,对照组注射等体积生理氯化钠,连续14 d。末次注射后24 h处死动物,分离肝细胞的细胞核、线粒体和微粒体3种亚细胞结构作傅里叶红外光谱(ATR-FTIR)分析。结果与对照组比较,各染氟组3种亚细胞结构在1 650～930 cm-1各峰值均降低(P<0.05),呈剂量-效应关系,敏感度依次为:微粒体>线粒体>细胞核;主成分分析显示:染氟使细胞核化学成分磷酸化增加、线粒体及微粒体多种化学成分改变。结论本实验条件下,ATR-FTIR检测到氟致肝细胞核、线粒体、微粒体的化学成分发生改变;ATR-FTIR有望作为细胞毒性反应的早期监测方法之一。
Objective To analyze the influence of fluoride on experimental animals＇ subeellular structure with ATR-FTIR. Methods 32 SPF Kunming mice were randomly divided into 4 groups. Mice in the contamination group were intraperitoneally given sodium fluoride injection at doses of 2, 8, 32 mg （kg d） -i respectively for 14 days, and the control group was given normal saline. 24 h after the final injection, hepatocyte nuclei, mitochondria and mierosome were separated to analyze with ATR- FTIR. Results The ATR-FTIR spectra showed that, the absorption peaks of 3 subcellular structures in fluorine groups at 1 650 - 930 em - i were significantly decreased （ P 〈 0. 05 ） compared with those of the control group. The decline showed dose- effect relationship and the sequence of sensitivity was microsome 〉 mitoehondrion 〉 nucleus. The principal component analysis showed that fluoride concentration was related to phosphorylation in nucleus and some chemical composition changes in mitochondri- a and mierosome. Conclusion In this experiment, contaminating fluoride can change chemical composition in hepatocyte nuclei, mitochondria and mierosome which were detected by ATR-FTIR analysis. ATR-FTIR may be used as an early monitoring method for cell toxicity reaction.
出处
《中国职业医学》
CAS
北大核心
2013年第1期6-9,共4页
China Occupational Medicine
基金
广西教育厅桂教科(2002316号)
