摘要
目的观察胞二磷胆碱结合康复训练对脑缺血大鼠运动功能及半暗带区存活素（survivin）表达的影响。方法成年雄性sD大鼠120只，采用Longa线栓法制作大鼠左侧大脑中动脉闭塞（MCAO）模型，选出造模成功后符合标准的大鼠96只随机分为对照组、药物组、康复训练组和药物结合康复训练组4组，每组大鼠24只。造模成功后第3天，对照组不做任何干预，药物组给予胞二磷胆碱（每日500mg／kg体重），康复训练组进行滚笼、平衡木、转棒、网屏等训练，药物结合康复训练组同时采用药物组和康复训练组的方法综合治疗。4组大鼠均于造模后第7、14、21天进行神经行为学综合评分，并取脑组织采用免疫组化法观察缺血周围皮质survivin的表达。结果造模后第7，14，21天，药物组神经行为学评分与对照组同时间点比较，差异均无统计学意义（P〉0．05）；造模后第14，21天，康复训练组、药物结合康复训练组的神经行为学评分显著优于对照组和药物对照组同时间点（P〈0．05），且药物结合康复训练组造模后第14，21天的神经行为学综合评分分别为（2．75±0．50）分，（0．75±O．50）分，与康复训练组同时间点比较，差异均有统计学意义（P〈0．05）。造模后第7，14，21天，药物组、康复训练组和药物结合康复训练组survivin的表达水平与对照组同时间点比较，差异均有统计学意义（P〈0．05）；药物结合康复训练组survivin的表达水平分别与药物组和康复训练组同时间点比较，差异亦有统计学意义（P〈0．05）。结论胞二磷胆碱结合合理有序的康复训练能明显提高脑缺血大鼠运动功能的恢复，综合疗法效果更显著，其机制可能与缺血周围皮质survivin表达上调有关。
Objective To investigate the effect of drugs in combination with rehabilitative training on motor function and the expression of survivin in the ischemic penumbra after focal cerebral ischemia. Methods One hun- dred and twenty male, adult, Sprague-Dawley rats were subjected to left middle cerebral artery occlusion （MCAO） by suturing. Ninety-six of them were then randomly divided into a control group, a drug group, a rehabilitative train- ing group, and a drugs in combination with rehabilitative training group, with 24 in each. For three days the rats in the control group received no treatment, while those in the drug group received 500 mg/kg of citicoline daily, those in the rehabilitative training group received motor training including balancing, grasping, rotating and walking exercises, and those in the drug and rehabilitative training group received both citicoline and the motor training. Behavioral tests were administered to all groups, and immunohistochemistry was used to detect the expression of survivin in the ische- mic penumbra. Results Average behavior scores in the drug group and the control group were not significantly dif- ferent at day 7, 14 or 21 after the MCAO. Average behavior scores in the rehabilitative training and drug in combina- tion with rehabilitative training groups were significantly superior to those of the control and drug groups at day 14 and 21. At those time points the average scores in the drug in combination with rehabilitative training group were also sig- nificantly better than those of the rehabilitation training group. Compared with control group, at the 7th, 14th and 21st day after MCAO, expression of survivin in the other three groups had increased significantly. Expression of sur- vivin in the group where drug treatment was combined with rehabilitative training was significantly greater than in the drug and rehabilitative training groups. Conclusions Citicoline in combination with rehabilitative training can ira-prove the recovery of motor function in rats significantly better than the other approaches. This might be partially at- tributed to the up-regulation of survivin expression in the peri-isehemic cortex.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2013年第2期86-90,共5页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
脑缺血
胞二磷胆碱
康复训练
存活素
Cerebral isehemia
Citieoline
Rehabilitation
Survivin
