期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

一步酶法生产7-氨基头孢烷酸的研究进展 被引量：8

Research progress in one-step enzymatic conversion of cephalosporin C to 7-aminocephalosporanic acid
在线阅读 下载PDF
导出
摘要 7-氨基头孢烷酸是合成头孢类抗生素的重要中间体,一般由头孢菌素C通过化学法或生物酶法裂解脱去侧链分子制备得到。综述了头孢菌素C酰化酶的来源、特性及其基因的克隆和改造,表达与纯化,固定化及其催化工艺的研究,并展望了一步酶法生产7-氨基头孢烷酸的应用前景。 7-aminocephalosporanic acid, an important intermediate for the preparation of semi-synthetic eephalosporin antibiotics, is generally produced by hydrolysis of cephalosporin C via chemical or enzymatic methods. The research progress in one-step enzymatic conversion of eephalosporin C to 7-aminoeephalosporanic acid, involving research background,origin, properties, gene cloning and evolution of eephalosporin C aeylase, expression and purification of eephalosporin C acylase, enzyme immobilization and catalysis, as well as the application prospects of the novel process, is reviewed. The application prospects of one-step enzymatic conversion of cephalosporin C to 7-aminocephalosporanic acid are out forward as well.
作者 姚舜 罗晖 常雁红 于慧敏 李强 沈忠耀
机构地区 北京科技大学化学与生物工程学院 北京科技大学土木与环境工程学院 清华大学化学工程系
出处 《现代化工》 CAS CSCD 北大核心 2013年第2期11-14,16,共5页 Modern Chemical Industry
基金 国家自然科学基金项目(21276023) 国家"863"计划项目(2008AA02Z207) 中央高校基本科研业务费专项资金(FRF-BR-11-002A) 北京市科技新星计划(2006B22)
关键词 7-氨基头孢烷酸 两步酶法 一步酶法 头孢菌素C酰化酶 7-ACA two-step enzymatic process one-step enzymatic process CPC acylase
分类号 TQ9 [化学工程]
  • 相关文献

参考文献32

  • 1Parmar A,Kumar H,Marwaha S S. Recent trends in enzymatic conversion of cephalosporin C to 7-aminocephalosporanic acid (7-ACA)[J].Critical Reviews in Biotechnology,1998,(01):1-12.
  • 2Daniele B,Rossella B,Paolo G. Enzymatic transformation of cephalosporin C to 7-ACA by simultaneous action of immobilized D-amno acid oxidase and glutaryl-7-ACA acylase[J].Applied Biochemistry and Biotechnology,1998,(2/3):257-268.
  • 3Luo H,Li Q,Yu H M. Construction and application of fusion proteins of D-amino acid oxidase and GL-7-ACA acylase for direct bioconversion of cephalosperin C to 7-ACA[J].Biotechnology Letters,2004,(11):939-945.
  • 4罗晖,李强,童忆舟,司颖涛,沈忠耀.酶法生产7-氨基头孢烷酸的研究进展[J].现代化工,2002,22(12):18-22. 被引量：24
  • 5Tan Q,Song Q X,Wei D Z. Single-pot conversion of cephalosporin C to 7-aminocephalosporanic acid using cell-bound and supportbound enzymes[J].Enzyme and Microbial Technology,2006,(05):1166-1172.
  • 6Shin Y C,John J Y,Jung K H. Cephalosporin C acylase mutant and method for preparing 7-ACA using same[P].US,7592168B2,2009.
  • 7Shibuya Y,Matsumoto K,Fujii T. Isolation and properties of 7β-(4-carboxybutanamido) cephalosporanic acid acylase-producing bacteria[J].Agricultural and Biological Chemistry,1981,(07):1561-1567.
  • 8Aramori I,Fukagawa M,Tsumura M. Isolation of soil strains producing new cephalosporin acylases[J].Journal of Fermentation and Bioengineering,1991,(04):227-231.
  • 9Binder R G,Numata K,Lowe D A. Isolation and characterization of a Pseudomonas strain producing glutaryl-7-aminocephalosporanic acid acylase[J].Applied and Environmental Microbiology,1993,(10):3321-3326.
  • 10Matsuda A,Matsuyama K,Yamamoto K. Cloning and characterization of the genes for two distinct cephalosporin acylase from a Pseudomonas strain[J].Journal of Bacteriology,1987,(12):5815-5820.

二级参考文献51

  • 1徐剑文,林建银,林旭,齐元麟,张晓艳.蛇毒金属蛋白酶抑制剂基因的合成及杆状病毒表达载体的构建[J].中国生物医学工程学报,2006,25(6):755-764. 被引量：7
  • 2Pollegioni L, Lorenzi S, Rosini E, et al. Evolution of an acylase active on cephalosporin C [J]. Protein Sci, 2005, 14(12) : 3064 - 3076.
  • 3Bora O, Myungsook K, Jongchul Y, et al. Deacylation activity of cephalosporin acylase to cephalosporin C is improved by changing the side-chain conformations of active site residues [J]. Biochem Biophys Res Commun, 2003, 310: 19-27.
  • 4Li Y, Chen J F, Jiang W H, et al. In vivo post translational processing and subunit reconstitution of cephalosporin acylase from Pseudomonas sp. 130 [J]. Eur J Biochem, 1999, 262(3): 713-719.
  • 5Saito Y, Fujimura T, Ishii Y, et al. Oxidative Modification of a Cephalosporin C Acylase from Pseudomonas .Strain N176 and Site Directed Mutagenesis of the Gene [J]. Appl Envir Microb, 1996, 62(8): 2919-2925.
  • 6Young L, Dong Q. Two-step total gene synthesis method [J]. Nucleic Acids Res, 2004, 32(7):e59.
  • 7Xiong A S, Yao Q H, Peng R H, et al. A simple, rapid, high-fidelity and cost-effective PCR based two step DNA synthesis method for long gene sequences [J]. Nucleic Acids Res, 2004, 32(12): e98.
  • 8Hoover D M, Lubkowski J. DNAWorks: An automated method for designing oligonucleotides for PCR-based gene synthesis [J]. Nucleic Acids Res, 2002, 30(10): e43.
  • 9Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual [M]. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989.
  • 10Kim D W, Yoon K H. Cloning and high expression of glutaryl 7-aminocephalosporanic acid acylase gene from Pseudomonas diminuta. Biotechnol Lett, 2001, 23: 1067- 1071.

共引文献33

同被引文献110

引证文献8

二级引证文献20

现代化工
2013年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部