摘要
目的:比较Vero细胞在不同的商品化微载体中固定化培养的生长和代谢。方法:以Vero细胞在含1%新生牛血清的DMEM/F12中培养的细胞形态、活细胞密度和细胞活力为指标,考察Vero细胞在2D MicroHex、Biosilon、Cytodex1和Cytopore1微载体固定化培养的细胞生长;以葡萄糖比消耗速率(qglc)、乳酸比生产速率(qlac)、谷氨酰胺比消耗速率(qgln)和谷氨酸比生产速率(qglu)为指标,考察Vero细胞在不同微载体固定化培养的细胞代谢。结果:Vero细胞在2DMicroHex、Biosilon、Cytodex1和Cytopore1微载体固定化培养7d的活细胞密度分别为18.4×105cells/ml、21.9×105cells/ml、23.9×105cells/ml和16.2×105cells/ml,生长在Cytodex1表面的Vero细胞分布均匀、形态清晰;Vero细胞在不同微载体固定化培养的代谢指标基本相同。结论:Vero细胞在Cytodex1微载体固定化培养的效果优于其它商品化微载体,可作为目前用于病毒疫苗生产的Vero细胞固定化培养的首选微载体。
Objective：To investigate the growth and metabolism of Vero cells immobilized cultured on various microcarriers.Methods：Cell viability and cell morphology of the Vero cells cultured in 1%（v/v） newborn bovine serum DMEM/F12,the growth of the Vero cells cultured on 2D MicroHex,Biosilon,Cytodex 1 and Cytopore 1 was evaluated on the viable cell density;The specific consumption rate of glucose（qglc）,the specific production rate of lactate（qlac）,the specific consumption rate of glutamine（qgln）,and the specific consumption rate of glutamate（qglu） were used as the evaluation indexes,the metabolism of the Vero cells cultured on various microcarriers was determined.Results：After 7 d in culture the viable cell density of the Vero cells cultured on 2D MicroHex,Biosilon,Cytodex 1 and Cytopore 1 was 18.4×105 cells/ml,21.9×105 cells/ml,23.9×105 cells/ml and 16.2×105 cells/ml,respectively.And,the Vero cells growth on Cytodex 1 formed compact cell layers with distinct cell morphology;Metabolic indexes of the Vero cells cultured on various microcarriers were basically the same.Conclusion：Among these microcarriers,Cytodex 1 is comparatively good for the growth of Vero cells,and could be used as the favor choice of microcarrier for the large-scale cultivation of Vero cells for viral vaccine production.
出处
《现代生物医学进展》
CAS
2012年第36期7001-7004,共4页
Progress in Modern Biomedicine
基金
"重大新药创制"国家重大科技专项课题(2011ZX09401-019)
