摘要
根据GeneBank上红色原鸡的MHCB-G序列设计特异性引物,利用PCR-SSCP和测序方法对文昌鸡18只个体的MHCB-G基因第2外显子序列332bp区域进行序列多态性分析。通过PCR-SSCP分型得到16种基因型;测序检测到21个变异位点,其中20个位点导致16个氨基酸位点发生变异。结果表明:所有序列均未发生长度变化;文昌鸡群体内MHCB-G的多态性较丰富,主要体现在氨基酸多态性上,与其抗原多样性有密切关系。
The sequence polymorphisms of the second exon of major hiscompatibility complex （MHC） B-G of 18 individuals in Hainan Wenchang Chicken were detected by the method of PCR-SSCP and direct sequencing with primers which were designed based on the sequence of Gallus gallaus in GenBank. These chickens were grouped into 16 genotypes by PCR-SSCP. Sequencing detected 21 polymorphic sites, 20 sites of which cause 16 amino acids sites to mutate among the individuals. The results indicated that the length of MHC B-G sequences in these individuals has no variation. Wenchang Chicken population has rich polymorphisms mainly in the amino acids,which are closely related with the diversity of MHC B-G antigen.
出处
《热带生物学报》
2012年第4期314-317,共4页
Journal of Tropical Biology
基金
海南省自然科学基金项目(310036)
海南省教育厅高等学校科学研究项目(Hjkj2011-11)
海南大学青年基金项目(qnjj1017)
海南大学科研启动项目(Kyqd1116)
关键词
文昌鸡
MHC
B-G
第2外显子
多态性
：Wenchang chicken
major hiscompatibility complex
second exon
polymorphism
