摘要
目的研究microRNA-21（miR-21）、PTEN、NF-κB、Caspase-9在结肠癌中的表达及在肿瘤进展中的可能机制。方法检测结肠癌、结肠腺瘤及正常黏膜中上述指标的表达及凋亡细胞百分率。结果miR-21、PTEN、NF-κB、Caspase-9阳性表达率在正常黏膜中分别为0．39±0．02、50％、25％、50％，在结肠腺瘤中分别为0．62±0．06、50％、35％、55％，在结肠癌中分别为0．88±0．04、30％、75％、20％。凋亡细胞百分率分别为（24．64±7．66）％、（15．86±1．44）％、（6．20±2．57）％（均P〈0．05）。miR-21与TNM分期、浸润深度、分化程度及NF-κB表达呈正相关，与PTEN、Caspase-9表达及凋亡细胞百分率呈负相关（r=0．647、0．297、0．259、0．519、-0．299、-0．388、-0．931，P均〈0．05）。结论结肠癌中miR-21高表达可能通过下调PTEN、Caspase-9、上调NF-κB、从而抑制细胞凋亡参与肿瘤进展。
Objective To investigate expressions of microRNA-21 （miR-21）, FFEN, NF-κB, and caspase-9 in colon carcinoma and their possible mechanisms in progression of tumor. Methods Expressions of above-mentioned indexes and percentage of apoptotic cells in colon carcinoma, colon adenoma, and normal tissue specimen were detected. Results Expressions of miR-21, PTEN, NF-κB, and caspase- 9 in normal tissue were 0. 39 ±0.02, 50% , 25% , and 50%. Expressions of them in colon adenoma were 0. 62 ±0. 06, 50%, 35% , and 55% , and those in colon carcinoma were 0. 88 ±0. 04, 30% , 75% , and 20%. Percentage of apoptotic cells were （ 24. 64 ± 7.66） %, （ 15.86± 1.44 ） % , and （ 6. 20± 2. 57 ） % , respectively. The above P-values were all less than 0.05. Expression of miR-21 was positively correlated with TNM stage, depth of infiltration, differentiated level and expression of NF-κB while inversely correlated with PTEN and caspase-9 expressions and percentage of apoptotic cells. The r-values were 0. 647, 0. 297, 0. 259, 0. 519, -0. 299, - O. 388, and - 0. 931, respectively. Conclusions Hyperexpression of miR- 21 participated in progression of colon carcinoma probably through down-regulating expressions of PTEN and caspase-9 and up-regulating expression of NF-κB and then suppressing cell apoptosis.
出处
《中国医师杂志》
CAS
2013年第1期39-41,共3页
Journal of Chinese Physician
