摘要
目的评价BACTEC MIGT960快速检测8种抗结核药物敏感性效果。方法收集住院结核病人痰标本分离的结核分枝杆菌菌株,分别采用L-J比例法和MGIT960法进行药物敏感性检测,在完成一线4种药物的药敏检测后,选取对任何一种一线药物(异烟肼、利福平、链霉素和乙胺丁醇)耐药的菌株进行二线药物(卷曲霉素、卡那霉素、氧氟沙星和乙硫异烟胺)敏感性检测。从结果报告时间和符合率等方面进行评价。统计学分析采用kappa检验和t检验,P值为0.05。结果对212株结核分枝杆菌临床分离株进行了4种一线药物的药物敏感性检测,随后,对耐任何一种一线药物的90株菌进行了4种二线药物的药物敏感性检测,以L-J比例法为金标准,MGIT 960法的准确度和Kappa值分别为:异烟肼96.0%和0.92,利福平99.0%和0.98,链霉素99.0%和0.97,乙胺丁醇96.0%和0.81,卷曲霉素100.0%和1.00,卡那霉素99.0%和0.85,氧氟沙星96.0%和0.88,乙硫异烟胺94.0%和0.73;完成一线/二线药物药敏试验的平均时间,MGIT 960为8.10和10d,L-J法则均为30d,两种方法培养时间的差异有统计学意义(P<0.05)。结论 MGIT 960法与传统的L-J比例法药敏试验比较,具有符合率较好、检测时间明显缩短的特点,有利于临床及时有效合理化用药。
The purpose of this study was to evaluate the effects of BACTEC MGIT 960 for detecting the sensitivity of 8 drugs against Mycobacteriurn tuberculosis （M. tuberculosis）. The M. tuberculosis clinical strains were isolated from sputum specimens of the patients with tuberculosis （TB） in the Hunan Provincial Chest Hospital. Drug sensitivity test （DST） of 8 drugs was conducted by means of L-J proportional method and MGIT960 method respectively. The 4 first-line drugs used in this study were Isoniazid （INH）, Rifampicin （RFP）, Streptomycin （SM）, and Ethambutol （EMB）, and the 4 second-line drugs were Capreomycin （CPM）, Kanamycin （KAN）, Ofloxacin （OFLX）, and Ethionamide （ETH）. After the susceptibility test for the 4 first-line drugs were conpleted, the strains which were drug-resistant to any kind of the 4 first line drugs were se- lected for testing the susceptibility of the 4 second drugs. Statistics analysis was conducted by Kappa and t tests~ and the P value for significance was 0.05. The sensitivity to the 4 first-line drugs of 212 clinincal M. tuberculosis isolates was tested. And then, the 90 strains which were resistant to any kind of the 4 first-lin drugs were tested for their sensitivity to the 4 sec ond-line drugs. Compared with the results of L-J proportional method, the accuracy rates and the Kappa values of the results with MGIT 960 method for testing the sensitivity to INH, RFP, SM, EMB, CPM, KAN, OFLX, and ETH were 96.0% and 0. 92, 99. 0% and 0. 98, 99. 0%and 0. 97,drugs with MGIT 960 and L-J proportional method were 8.10 and 10 days, 30 and 30 days, respectively. There were signifi- cant difference among them （P（0.05）. It＇s suggested that MGIT 960 is better than L-J proportional method in testing the susceptibilities of M. tuberculosis to the first- and the second-line drugs for its accuracy and shorter testing time. MGIT 960 is time-saving and effective for rational use of drugs in clinical.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2013年第2期166-169,共4页
Chinese Journal of Zoonoses
基金
卫生部国际交流与合作中心--碧迪结核病控制项目(No.MOH-BD-2-3)资助~~
关键词
MIGT960
比例法
结核分枝杆菌
药敏检测
BACTEC MGIT 960 Mycobaeterium tuberculosis
L J. proportional method ~ drug susceptibility test
