摘要
从造纸废水处理厂卡鲁赛尔(Carrousel)氧化沟的活性污泥样品中分离到了1株产絮凝剂的菌株B-6,经生理生化试验和16Sr DN A基因序列分析,鉴定为芽胞杆菌属(Bacillus sp.)。该菌株产生的絮凝剂具有良好的酸碱稳定性,在pH值1~5和7~11范围内,其絮凝活性维持在80%以上。优化该菌发酵上清液的絮凝条件,结果表明,加入5 mL的1%CaCl2,发酵上清液投加量为0.8 mL时,该菌株发酵上清液对高岭土悬浊液的絮凝率可达95.4%。
A bioflocculant-producing strain, named B -6, was isolated from Carrousel oxidation ditch activated sludge and identified as Bacillus sp. by 16S rDNA sequence. The biochemical and physiological characteristics of the strain were studied. The bioflocculant was pH stable, the flocculating activity was over 80% in the range of pH 1 - 5 and 7 - 11. 0. 8 mL the bacterium fermentation broth showed the maximum flocculating activity of 95.4% in 5.0 g/L Kaolin suspension containing 5 mL 1% CaCl2.
出处
《生物学杂志》
CAS
CSCD
2013年第1期91-94,共4页
Journal of Biology
基金
湖南省教育厅科研项目(11C0988)
怀化学院科研项目(HHUY2011-11)