摘要
目的探讨rhBNP对动脉粥样硬化(AS)大鼠模型血管内皮功能的影响。方法 40只健康wistar大鼠,随机分为对照组、模型组、处理组各8只。处理组分别给予硝酸酯类、阿魏酸钠、重组人脑利钠肽皮下注射。采用酶联免疫吸附(ELISA)方法测定各组大鼠处理前后血清中血栓素B2(TXB2)和6-酮前列腺素F1a(6-Keto-PGF1a)的含量,并且光镜下观察各组处理前后大鼠的主动脉内膜细胞的变化。结果 1)处理组药物治疗后,血清中TXB2均下降、6-Keto-PGF1a均升高,与治疗前比较有统计学差异(P<0.05);2)各处理组间药物应用后二者无差别(P>0.05);3)光镜的观察结果提示:模型组血管管腔内有附壁斑块;平滑肌细胞排列紊乱;处理组内膜较光滑,局部有MC粘附,偶可见EC缺失,但好于模型组,各处理组之间未见明显差别。结论 rhBNP通过保护血管内皮功能具有抗动脉粥样硬化的作用。
Objective The effects of Brain Natriuretic Peptide on the function of rats arteriosclerosis model vascular endothelial. Methods 40 healthy wistar rats,divided into five groups at random, eight rats in contorl group, eight in model group, each of the treatment group of nitrates （ H ua ren xin shu）, Sodium Ferulate （ Haisi）, rhBNP （xinhuosu） model group were eight rats. Adopt enzyme-linked immunosorbent adsorption （ELISA） methods to determine each rat treatment and serum blood TXB2 and 6-Keto-PGFla content,and light-rrlicroscopy each rat before and after processing the aortic lining cells changes. Results a. The treatment group serum TXB2 and 6-Keto-PGFla levels obviously differ- ent than before（P〈0.05）, b. The treatment of each group in serum TXB2 and 6-Keto-PGFla content is difference（P〉0.05）. c. Optical microscope observations, the control group vascular lumen, no calcium sediment, big plaques can be seen. Smooth muscle cells is boom and ectoblast is conjunction tissue, arranging disorganized. The treatment group is smoother,local macrophage adhesion, visible EC missing, but better than model group, and the treatment group showed no significant difference between. Conclusion rhBNP can not only increases the content of PGI2 arterial stiffness rat models,lower TXA2 level,but also atherosclerosis rats damage,vascular endothelial protecting endothelial function.
出处
《中国实验诊断学》
2013年第2期247-251,共5页
Chinese Journal of Laboratory Diagnosis
