摘要
本研究中分别对来自西宁动物园的21种观赏动物,乌兰县的16头牦牛和42只山羊,用免疫荧光法(IFT)进行了隐孢子虫卵囊的调查。用套式PCR进行种和基因型的检测,并且用18SrRNA进行序列分析。用IFT分别在西宁动物园的17个观赏动物,乌兰县的2头牦牛和15只山羊中发现了隐孢子虫的卵囊。对IFT阳性样品进行PCR扩增,其中10份为PCR阳性。并且用黑豹(Panthera pardus)、黑颈鹤(Grus nigricollis),蛮羊(Ammotragus lervia),羚牛(Budorcastaxicolor),小熊猫(Ailurus fulgens)和白马鸡(Crossoptilon crossoptilon)等动物隐孢子虫卵囊的PCR序列分析结果跟与微小隐孢子虫鼠基因型进行了比较。本研究首次报道了青海省野生动物中隐孢子虫的感染情况,并且首次在北山羊上分离得到的隐孢子虫中发现了鹿源基因型,类似C.bovis基因型,以及在山羊和野牦牛上发现了新的Cryptosporidium spp。
The presence of Cryptosporidium oocysts of 21 zoo animals in the Xining Zoo, 16 farm yaks and 42 farm goats in wulan county Qinghai province, were investigated by an immunofluoresce -nce test genotypes were determined by nested polymerase chain reaction （nPCR）and sequence analysis （IFT）. The of a fragment species and of the small subunit（SSU） rRNA gene. Cryptosporidium oocysts were found in 17 zoo animals, 2 yaks, and 15 goats by IFT. The IFr positive samples were further investigated by PCR, and 10 of them were found to be positive by that method also. Sequence analysis of the PCR products derived from Cryptosporidium oocysts from Black leopard （ Panthera pardus ）, Heijing He （ Grus nigricollis }, Barbary sheep （ Ammotragus lervia }, Takin （ Budorcas taxicolor }, Lesser panda （ Ailurus fulgens）, and White -eared pheasant （Crossoptilon crossoptilon） fecal samples matched that of Cryptosporidium mouse genotype. The present work reports the first data on Cryptosporidium infections in animals from the parvum Qinghai province and the first findings of the ＇cervine＇ genotype in Capra ibex, C. bovis - like genotype and the new Cryptosporidium spp. in farm goat and in wild yak.
出处
《青海畜牧兽医杂志》
2013年第1期1-3,共3页
Chinese Qinghai Journal of Animal and Veterinary Sciences
基金
农业部948专项(2009-Z48)项目名称:三江源地区饮用水中贾第鞭毛虫及隐孢子虫病的检测技术的引进及示范
关键词
动物
隐孢子虫
分子特征
Animal, Cryptosporidium, Molecular characterization
