摘要
目的建立HPLC法测定孕康糖浆中补骨脂素和异补骨脂素含量的方法。方法采用高效液相色谱法测定。色谱柱:Agilent C18柱,流动相:甲醇-水(40∶60),流速:1.0 ml.min-1,检测波长:246 nm。结果补骨脂素在0.0334～0.334μg(r=0.9999),异补骨脂素在0.047 5～0.475μg(r=0.999 9)之间线性关系良好;补骨脂素的平均回收率为98.31%,RSD=1.49%;异补骨脂素的平均回收率97.30%,RSD=1.18%。结论试验表明,该方法操作简单,分离效果好,灵敏度高,可以用于孕康糖浆的质量控制。
Objective To establish an HPLC method for the determination of psoralen and isopsoralen in Yunkang Syrup.Methods Agilent C18column was used.The mobile phase was methanol-water（40∶ 60）.The flow rate was 1.0 ml·min-1.The detection wavelength was 246nm.Results The calibration curves of psoralen and isopsoralen were linear at the range of 0.033 4~0.334 μg（r=0.999 9）,0.047 5~0.475 μg（r=0.999 9）respectively.The average recoveries were 98.31% for psoralen（RSD=1.49%） and 97.30% for psoralen（RSD=1.18%）.Conclusion The method is simple and sensitive with good separation efficiency,which can be used for routine quality control of yunkang syrup.
出处
《安徽医药》
CAS
2012年第8期1088-1089,共2页
Anhui Medical and Pharmaceutical Journal
