摘要
目的探讨miR-146a含量变化在甲状腺乳头状癌（PTC）发生发展过程中的作用及意义。方法应用荧光定量聚合酶链反应（polymerasechainreaction，PCR）检测43例PTC、36例结节性甲状腺肿组织的miR-146a含量变化及32例PTC、23例结节性甲状腺肿患者外周血的miR-146a含量变化，并根据PTC患者主要临床病理参数进行分析。结果①PTC与结节性甲状腺肿组织中miR-146a相对含量分别为0．0280±0．0131和0．0212±0．0111，差异有统计学意义（P〈0．05）；PTC组织的miR-146a含量变化与患者淋巴结转移、肿瘤分期等临床病理参数有明显关系（P〈0．05），与患者年龄、肿瘤大小等无关（P〉0．05）。②PTC与结节性甲状腺肿外周血中miR-146a相对含量分别为0．0891±0．0419和0．09224-0．0456，2者差异无统计学意义（P〉0．05）；PTC外周血的miR-146a含量变化与患者年龄、肿瘤大小、淋巴结转移、肿瘤分期等无相关性（P〉0．05）。结论miR-146a在PTC中表达升高，与淋巴结转移、肿瘤分期等临床病理参数有明显关系，多见于进展期，提示miR-146a在PTC的发生发展过程中起-定作用。
Objective To study the change of miR-146a in the development and progression of papillary thyroid carcinoma（PTC）. Methods Real-time fluorescence quantitative polymerase chain reaction was used to detect the change of miR-146a in tumor tissues of 43 cases of PTC and 36 cases of nodular goiter, also in peripheral blood of 32 cases of PTC and 23 cases of nodular goiter. The correlation of miR-146a change and the clinicopathological parameters of patients with PTC was analyzed. Results ① The relative content of miR-146a in the 43 patients with PTC and 36 patients with nodular goiter was 0. 0280 ± 0. 0131 and 0. 0212 ± 0. 0111 respectively. The difference had statistical significance （ P 〈 0. 05 ）. miR-146a in PTC tissues was significantly correlated to elinicopathological parameters such as lymph node metastasis, tumor stage （ P 〈 0. 05 ）, and it was unrelated to age or tumor size（P 〉0.05）. ② The relative content of miR-146a in peripheral blood of 32 patients with PTC and 23 patients with nodular goiter was 0. 0891 ± 0. 0419 and 0. 0922 ± 0. 0456 respectively. The difference had no statistical significance（ P 〉0. 05 ）. There was no significant correlation between miR-146a in peripheral blood of patients with PTC and age, tumor stage, lymph node metastasis, or tumor size of patients（ P 〉 0. 05）. Conclusions MiR-146a expression is upregulated in patients with PTC, and there is a significant correlation between miR-146a content and clinicopathological parameters such as lymph node metastasis, tumor stage, especially in advanced stage. It suggests that miR-146a may play a role in carcinogenesis and development of FFC.
出处
《中华内分泌外科杂志》
CAS
2013年第1期56-59,共4页
Chinese Journal of Endocrine Surgery
