摘要
目的制备靶向A型H1N1流感病毒RNA聚合酶(PA)基因的小干扰RNA(siRNA),研究其抑制病毒复制的效果。方法设计并合成3对靶向A型H1N1流感病毒PA基因的siRNA,构建表达质粒pS-PA646、pS-PA841、pS-PA1537,分别转染MDCK细胞及鸡胚,并感染H1N1亚流感病毒,检测siRNA抑制流感病毒复制的效果。结果设计的3对siRNA中,pS-PA1537可明显抑制A型H1N1流感病毒在MDCK细胞及鸡胚中的复制。结论该研究为开发抗H1N1治疗制剂研究奠定了基础。
Objective To establish the small interfering RNA(siRNA) targeting RNA polymerase(PA) gene of influenza A(H1N1) virus to study its effect of inhibiting virus replication.Methods The three pairs of siRNA targeting PA gene of influenza A(H1N1) virus were designed and synthesized,as well as constructed expression plasmid pS-PA646,pS-PA841 and pS-PA1537,being transfected into MDCK cells and chicken embryos respectively and infected with influenza virus subtype H1N1,to detect effects of siRNA on inhibiting influenza virus replication.Results In the designed 3 pairs of siRNA,pS-PA1537 inhibited the replication of influenza A(H1N1) virus in MDCK cells and chicken embryos.Conclusions The study lays the foundation for the development of therapeutic agents resistant H1N1.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2013年第4期835-837,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金项目(2009ZX10004-103)
吉林省科技厅资助项目(20090487
201101066)
吉林大学研究生创新研究计划项目(20101035)
