摘要
目的:了解临床分离大肠埃希菌的耐药性和超广谱β-内酰胺酶(ESBLs)和头孢菌素酶(AmpC)基因分布情况。方法:采用VITEK-60全自动细菌分析仪对657株大肠埃希菌临床分离株进行药敏检测,并通过PCR方法检测其中140株试验菌的ESBLs和AmpC酶相关基因,对部分ESBLs基因进行DNA测序。结果:140株大肠埃希菌中ESBLs表型试验阳性的有86株,阳性率为61.4%。86株ESBLs表型阳性菌株中检测到ESBLs基因阳性的有80株,占93.0%。其中有61株携带CTX-M-9型基因,30株携带CTX-M-1型基因,23株携带TEM型基因,1株携带SHV型基因。140株大肠埃希菌中检测到AmpC酶基因阳性的有18株,阳性率为12.9%,其中有10株携带DHA型基因,9株携带CIT型基因,1株携带FOX型基因。结论:临床分离大肠埃希菌耐药情况严重,其携带ESBLs基因以CTX-M-9型为主,AmpC酶基因以DHA和CIT为主。
Objective：To understand the drug-resistance and the distribution of the genes of ESBLs and AmpC in Escherichia coli.Methods： Antimicrobial susceptibility was tested with Vitek60-AMS.Genotypes of ESBLs and AmpC were analysed with PCR and part of ESBLs genes were verified by DNA sequencing.Results： Eighty-six strains were found with ESBLs in 140 Escherichia coli isolates,and the positive rate was 61.4%.PCR was used to dectect the ESBLs genes of 86 strains,in which there were 80 stains with ESBLs genes and the positive rate was 93.0%.There were 61 strains with CTX-M-9,30 strains with CTX-M-1,23 stains with TEM,1 strain with SHV.PCR also was used to detect the AmpC genes of 140 Escherichia coli isolates,in which there were 18 stains with AmpC genes,and the positive rate was 12.9%.There were 10 stains with DHA gene,9 strains with CIT gene,1 strain with FOX gene.Conclusion： The multidrug resistance of Escherichia coli from clinical isolates is very serious.The major ESBLs genotype was CTX-M-9-like gene in our hospital.Among the AmpC genens,the major genotypes were DHA type and CIT type.
出处
《中国卫生检验杂志》
北大核心
2013年第1期17-20,共4页
Chinese Journal of Health Laboratory Technology
