摘要
目的分析福建省2010-2011年手足口病患者中肠道病毒71型(EV71)分离株的基因特征。方法从福建省2010年和2011年手足口病患者标本中分离到EV71病毒74株(2010年50株,2011年24株),用RT-PCR法扩增VP1区并测序,利用DNAStar和MEGA4.0分析软件进行同源性分析和构建系统发生树。结果 74株病毒的核苷酸和氨基酸序列同源性分别为95.4%～100.0%和97.3%～100.0%;与安徽省阜阳市2008年流行株Fuyang17.08-2的核苷酸和氨基酸序列同源性分别为97.1%～99.3%和98.7%～100.0%,在系统进化树上与C4a亚型代表株处于同一分支,属于C4a基因亚型。2010年分离的4株EV71与阜阳代表株在SP70区域的氨基酸序列存在差异,而SP55区域的氨基酸序列则完全一致。结论福建省2010年和2011年手足口病患者中EV71的主要流行型别为C4a亚型,处于不同的进化链中,提示福建省可能存在多个传播链及部分EV71分离株具有进化趋势,对EV71的分子流行病学监测和分析有助于揭示病毒传播及进化规律。
Objective To characterize the genetic features of the enterovirus 71（EV71）strains recently isolated in Fujian province of China.Methods VP1 gene fragments of EV71 strains were amplified by RT-PCR and sequenced;nucleotide sequence alignment and phylogenetic analysis were done by using DNAstar and MEGA4.0 software.Results A total of 74 EV71 strains were isolated from 2010 to 2011 in Fujian,China.Sequence analysis for partial region of the VP1 gene of all 74 strains showed 95.4%-100.0% and 97.3%-100.0% homology in nucleotides and amino acids,respectively.Comparing to the epidemic strain in Fuyang,Anhui province during 2008,our strains share high homology with the Fuyang epidemic strain 17.08-2（97.1%-99.3% and 98.7%-100.0% in nucleotides and amino acids,respectively）.Phylogenetic analysis of the VP1 coding region of all 74 EV71 strains demonstrated that the EV71 isolates in Fujian are C4a genotype of EV71.Comparing to 4 representative strains isolated in Fuyang,Anhui province in 2010,difference were seen in SP70 region but not the SP55 region at the amino acid level.Conclusions The major epidemic strain of EV71 in Fujian province of China during 2010 and 2011 is C4a genotype.
出处
《中国病毒病杂志》
CAS
2013年第1期32-36,共5页
Chinese Journal of Viral Diseases
基金
国家"十二五"艾滋病和病毒性肝炎等重大传染病防治科技重大专项(2012ZX10004-210-003)
