摘要
以不同维生素K水平(0.13、2.15、3.25、6.40、12、17.20和23.20 mg/kg饲料)的7种精制饲料喂养初始体重约为(2.17±0.01)g的异育银鲫(Carassius auratus gibelio)10周,每个处理3个重复,研究异育银鲫对维生素K的需求量。结果显示:饲料中维生素K的添加可以明显降低摄食率,饲料中维生素K含量为2.15mg/kg时,摄食率出现最大值,之后显著下降(P<0.05),在12 mg/kg时达到最低值。特定生长率随着维生素K的添加表现出升高的趋势,饲料中维生素K含量为12 mg/kg时,出现最大值,但是差异不显著(P>0.05)。饲料中维生素K的含量从0.13 mg/kg升至3.25 mg/kg时,饲料效率显著升高(P<0.05),随着饲料中维生素K的进一步添加,趋于稳定(P>0.05),在12 mg/kg时达到最大值,并且与特定生长率呈正相关关系(SGR=0.01FE+0.95,R2=0.95)。血液红细胞数目随着饲料维生素K含量的增加先显著升高(P<0.05),在6.40 mg/kg时达到最大值,之后趋于稳定(P>0.05)。血红蛋白含量、血球容积比、血清钙含量与血液中红细胞数目表现出相似的趋势,均在不添加维生素K组出现最低值,但是差异不显著(P>0.05)。肝体比、肥满度及鱼体生化组成均不受饲料维生素K水平的影响(P>0.05)。分别对饲料效率、红细胞数目进行折线回归得出异育银鲫幼鱼对维生素K的最适需求量为3.73—6.72 mg/kg饲料。
A 10-week feeding experiment was conducted to determine vitamin K requirement of juvenile gibel carp（Carassius auratus gibelio）.Purified diets with seven levels（0.13,2.15,3.25,6.40,12,17.20 and 23.20 mg/kg diet） of supplemental vitamin K3（menadione sodium bisulfite,MSB） were fed to C.gibelio（mean weight 2.17±0.01 g）.Each diet was fed to triplicate groups of fish.The results showed that fish fed with the diet supplemented with 2.15 mg vitamin K/kg had significantly higher feeding rate（FR） than fish fed with diets supplemented with 12 mg vitamin K/kg（P0.05）.Specific growth rate（SGR） increased with increasing dietary vitamin K levels and reached the highest value when dietary vitamin K content was 12 mg/kg（P0.05）.Feed efficiency（FE） was the highest in fish fed with diet supplemented with ≥3.25 mg vitamin K/kg,intermediate with 2.15 mg vitamin K/kg supplemented diet and the lowest in the control group（P0.05）.FE reached the highest value when the dietary vitamin K content was 12 mg/kg（P0.05） and showed a linear correlation with SGR（SGR = 0.01 FE ＋ 0.95,R2 = 0.95）.The number of red corpuscles（RBC） in blood increased significantly with increasing dietary vitamin K level（P0.05） and reached the plateau at 6.40 mg vitamin K/kg.The Hb content,Hct and serum Ca content followed the same trend as RBC and were the lowest in gibel carp fed with diet without vitamin K supplementation（P0.05）.HSI,CF and whole body composition were not affected by dietary vitamin K level（P0.05）.Based on the broken-line regression between FE or RBC and dietary vitamin K level,we concluded that the adequate supplemental dietary vitamin K content for gibel carp was 3.73—6.72 mg/kg.
出处
《水生生物学报》
CAS
CSCD
北大核心
2013年第1期8-15,共8页
Acta Hydrobiologica Sinica
基金
现代农业产业技术体系建设专项资金(CARS-46-19)
国家973项目(2009CB118702)
公益性行业(农业)专项(201003020,201203015,201203083)
国家自然科学基金委员会基金(31123001)和FEBL(2009FBZ03)资助
关键词
异育银鲫
维生素K
饲料利用
血液指标
需求量
Gibel carp
Vitamin K
Feed utilization
Haemotological index
Requirement
