摘要
目的:以紫薯1号和农林47为实验材料,建立超高效液相色谱—三重四级杆串联质谱(UPLC-MS/MS)法,研究紫薯提取液中花青苷定性的分析方法。方法:UPLC-MS/MS法,ACQUITY UPLC BEH C18(2.1mm×50mm,1.7μm);柱温:40℃;流速:0.3mL/min;以6%甲酸水溶液和乙腈为流动相,采用电喷雾(ESI)正离子模式,用MRM模式监测。结果:紫薯1号和农林47提取液中花青苷组分分别为矢车菊素-3-葡萄糖苷和芍药素-3-葡萄糖苷。结论:该方法具有准确度高,重复性好、简单、快速等显著优点,可用于紫薯及其它作物中花青苷组分的测定。
Objective：Establish a qualitative approach method to analyse anthocyanin in purple sweet potatoes of Zishu No.1 and Nonglin 47 using UPLC-MS/MS.Methods：The analysis was carried out with ACQUITY UPLC BEH C 18 column（ 2.1mm × 50mm,1.7 μm） ; the column temperature was 40℃; flow rate was 0.3mL /min; 6% formic acid and acetonitrile were used as the mobile phase. An electrospray positive ion multiple reaction monitoring （ MRM） was used as a detector.Result：The main anthocyanin component of Zishu No.1 and Nonglin 47 was cyanidin-3-glucoside and peonidin-3-glucoside. Conclusion：The results showed that the method was accurate,good repeatability,convenient and rapid,which was suitable for the rapid determination of anthocyanin component in purple sweet potatoes and other kinds of crop.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第3期317-320,共4页
Science and Technology of Food Industry
基金
陕西省农推研发专项(2012SNJC159NTF-H1)
