摘要
目的:检测特发性红皮病患者石蜡包埋皮肤组织T细胞受体(TCR)γ基因重排。方法:提取特发性红皮病患者石蜡包埋组织DNA 28份,利用BIOMED-2系统TCRγ引物组合进行TCRγ基因重排检测,以28例银屑病患者石蜡包埋组织作为阴性对照。结果:特发性红皮病患者石蜡包埋组织TCRγ基因重排阳性率为7.1%,对照组阳性率为0。结论:部分TCRγ基因重排阳性特发性红皮病患者可发展成T细胞淋巴瘤。
Objective： To evaluate the values of TCRγ gene rearrangement detected by BIOMED - 2 multiplex tubes TCRγ （A ＋ B） in paraffin - embedded tissues from patients with idiopathic erythroderma. Methods： Twenty - eight DNA samples were extracted from paraffin - embedded multiplex tubes TCRγ （A ＋ B） in parattin - embedded tissues from patients with idiopathic erythroderma. TCRγ gene rearrangements were detected by BIOMED- 2 multiplex tubes TCRγ （A ＋ B） and comparison with 28 patients with psoriasis vulgaris was made. Results： The positivity rate of TCRγ gene rearrangements was 7.1% in samples from idiopathic erythroderma and none was positive in the patients with psoriasis vulgaris. Conclusion： Part of patients diagnosed as idiopathic erythroderma with positive clonal rearrangement of TCRγ gene may develop into T cell lymphoma.
出处
《中国麻风皮肤病杂志》
2013年第1期11-14,共4页
China Journal of Leprosy and Skin Diseases
