摘要
目的探讨电穿孔介导的摹因治疗对兔下颌骨牵引成骨过程中碱性成纤维细胞生长网子（basic fibroblast growth factor,bFGF）表达的影响。方法新西兰大白兔双侧下颌骨截骨，术后3d开始以0．8mm／d速度行下颌骨牵引，连续牵引7d，将实验动物分为：A、B、C、D、E5组。分别在牵引区注射2μg重组质粒pIRES-hVEGF165-hBMP2、pIRES-hBMP2、pIRES-hVEGF165、窄质粒pIRES及生理盐水。各组实验动物均施加电穿孔刺激。各组分别于固定期第7、14、28天处死动物取材行免疫组织化学榆查bFGF的表达，并利用病理图像分析系统进行分析。结果bFGF在肉芽中的成纤维细胞、单核巨噬细胞、多核巨噬细胞、间质细胞、成骨细胞和骨细胞中表达：1周时以C组表达较强．2、4周时以A、B、C、组表达仍较强，A、B、C组与D、E组相比差异有统计学意义（P〈0．01）。结论电穿孔介导的基因治疗能使bFGF在牵引区的表达增强和表达时限延长，发挥其生理作用，促进细胞的分裂增殖与分化及牵引区细胞基质的形成和新骨生成。这可能是基因治疗促进牵引区新骨生成的机制之一。
Objective To investigate the effect of gene therapy which was mediated by e ectro-poration on the expression of basic fibroblast growth factor （bFGF） duning mandible distraction. Methods Bilateral mandibular osteotomies were performed in New-Zealand rabbit. After a latency of 3 days, the mandibles were elongated using distractors with a rate of 0.8 mm/d for 7 days. The rab-bits were randomly divided into 5 groups： 2 μg recombinant plasmids pIRES-hVEGF165-hBMP2, pIRES-hBMP2, plRES-hVEGF165, pIRES and normal saline （NS） were injected into the distraction area of groups A, B, C, D and E, after completion of distraction, respectively. The lengthened man-dibles were harvested and processed for immunohistochemical detection of bFGF, and the mean optic densities and integral optical density of hFGF positive cells were measured by computerized image ana- lyzer. Results hFGF mainly located in fibroblasts, giant monocytes, polynuelear phagocytes, oseto-cytes, and osetoblasts in the connective tissue around bone tissue. The strongest expression was ob-served at the 7tb day, and weakened at 14th day of consolidation stage, there were no significant difference among groups A, B and C, at the 7th day of consolidation. However, there were significant differences between gene therapy groups （A, B and C） and control groups （D and E） （P〈0.01）. Conclusions C-ene therapy can enhance and prolong the expression of bFGF in distraction gap, which promotes the cell differentiation and proliferation, extracellular matrix synthesis and new bone forma-tion during distraction osteogenesis. This is probably one of the molecular mechanisms of the gene therapy promoting new hone formation in distraction gap.
出处
《中华医学美学美容杂志》
2013年第1期51-54,共4页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
国家自然科学基金资助项目（编号：30600653）,四川省教育厅重点项目（编号：11ZA239）,四川省卫生厅科研课题（编号：100285）
关键词
电穿孔
基因疗法
骨生成
碱性成纤维细胞生长因子
Electroporation
Gene therapy
Osteogenesis
Basic fibroblast growth factor（bFGF）
