摘要
目的:探讨17β-雌二醇对大鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)C反应蛋白(C-reactive protein,CRP)生成的影响及其相关机制。方法:采用免疫细胞化学法观察17β-雌二醇对正常状态大鼠VSMCs CRP表达的影响,免疫印迹法检测其对Ang-Ⅱ刺激大鼠VSMCs CRP及p-ERK1/2表达的影响,RT-PCR方法检测其对Ang-Ⅱ刺激状态大鼠VSMCs CRPmRNA表达的影响。结果:本实验所选用17β-雌二醇浓度(1、10、100 nmol/L)不影响正常状态大鼠VSMCs形态及CRP表达,但呈浓度依赖性抑制Ang-Ⅱ刺激的大鼠VSMCs CRP(r=-0.834,P<0.01)、p-ERK1/2(r=-0.712,P<0.05)及CRP mRNA(r=-0.856,P<0.01)的表达。结论:17β-雌二醇可以抑制Ang-Ⅱ刺激的大鼠VSMCs CRP及其mRNA表达,其作用机制与抑制ERK1/2信号转导通路相关。
Objective： To investigate the effects of 17β-estrogen on expressions of C-reactive protein （CRP） and its mRNA in vascular smooth muscle cells（VSMCs）. Methods： Immunocytochemistry was used to detect CRP level in normal VSMCs. The expressions of C-reactive protein and p-ERK1/2 in Ang -II -stimulated VSMCs were evaluated with Western blot. C-reactive protein mRNA was examined with RT -PCR. Results： 17β-estrogen had no effect on cell morphology and C-reactive protein expression in normal VSMCs; however, C-reactive protein and mRNA,as well as p-ERK1/2 were decreased in Ang-II -stimulated VSMCs after 17β-estrogen treatment in a concentration-dependent manner. Conclusion： 17β -estrogen may inhibit the expression of C-reactive protein and its mRNA in Ang- II-stimulated VSMCs via ERK1/2 signal transduction pathway in a concentration-dependent way.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2013年第1期86-91,共6页
Journal of Zhejiang University(Medical Sciences)
基金
西安医学院附属医院科研基金项目(XYFY10-24)
西安医学院科研基金项目(11FZ08)
关键词
RNA
信使
遗传
雌二醇
药理学
雌二醇
投药和剂量
肌
平滑
血管
药物作用
C反应蛋白质
药物作用
血管紧张素Ⅱ
药理学
丝裂原活化蛋白激酶1
代谢
信号传导
RNA, messenger/genetics
Estradiol/pharmacology
Estradiol/administration ＆ dosage
Muscle, smooth, vascular/drug effects
C-reactive protein/drug effects
Angiotensin II / pharmacology
Mitogen-activated protein kinase 1/metabolism
Signal transduction
