摘要
目的建立同时测定承德产及其他主产区柴胡中柴胡皂苷a和柴胡皂苷d的含量及其HPLC指纹图谱。方法采用Zorbax SB-C18色谱柱(250 mm×4.6 mm,5μm),乙腈溶液系统梯度洗脱,检测波长210 nm,流速1.0 mL.min-1,分析时间90 min。结果柴胡皂苷a和柴胡皂苷d的线性范围分别为33.2～531μg.mL-1(r=0.9999)、31.8～508μg.mL-1(r=0.9999);平均回收率分别为98.6%(RSD=0.9%)、98.9%(RSD=1.2%);15批药材的HPLC指纹图谱共确立6个共有峰。结论所用方法准确可靠,重复性好,为更好地控制柴胡的质量提供了科学依据。
OBJECTIVE To develop an HPLC method for the quantitative analysis of saikosaponin a and establish an HPLC fingerprint of Bupleurum chinense DC..METHODS The separation was carried out on Agilent ZORBAX SB-C18 column（250 mm×4.6 mm,5 μm） with acetonitrile and H2O as mobile phases in a gradient elution mode.The flow rate was 1.0 mL·min-1 and the detection wavelength was 210 nm.The analysis time was 90 min.RESULTS The linear ranges of saikosaponin a and d were 33.2-531 μg·mL-1（r=0.9999）,31.8-508 μg·mL-1（r=0.9999）,respectively.The average recovery rates of saikosaponin a and d were 98.6%（RSD=0.9%）,98.9%（RSD=1.2%）,respectively.In the fingerprint,6 common peaks were found from 15 batches of Bupleurum chinense DC..CONLUSION This method provides a scientific basis for controlling the quality of Bupleurum chinense.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2013年第1期86-88,共3页
West China Journal of Pharmaceutical Sciences
基金
2010年承德市科学技术研究与发展计划项目(编号:201021027)
