摘要
目的探讨基质相互作用分子1(STIM1)在高低转移性的乳腺癌细胞中的表达差异以及对钙库调节钙内流(SOCE)的影响。方法选取常见的高转移性乳腺癌细胞MDA-MB-231细胞与低转移性乳腺癌细胞MCF-7作为细胞模型,用划痕试验检测两种细胞的迁移能力,应用实时荧光定量PCR检测两种细胞中STIM1的mRNA表达,用Western blot检测其蛋白水平,用激光共聚焦显微镜检测两种细胞中SOCE的差异。结果划痕实验结果显示,MCF-7细胞在24 h和48 h的迁移细胞数都明显低于MDA-MB-231细胞24 h与48 h的迁移细胞数(P<0.01)。STIM1 mRNA在MCF-7细胞与MDA-MB-231细胞中的相对表达量分别为1.001 9±0.074 4和6.338 8±1.046 7,差异有统计学意义(P<0.05)。STIM1蛋白在MCF-7细胞与MDA-MB-231细胞中的相对表达量分别为0.530 2±0.177 8和1.296 8±0.075 8,差异有统计学意义(P<0.01)。MCF-7与MDA-MB-231细胞中SOCE引起的外钙内流相对比例分别为1.036 3±0.413 5和4.145 8±1.045 0,差异有统计学意义(P<0.01)。结论高转移性乳腺癌细胞中存在STIM1高表达和钙内流增高,这可能是其实现高转移性的机制之一。
Objective To investigate the differences of STIM1 expression and store - operated Ca2＋ entry （SOCE） between two breast cancer cells with different migration capability. Methods High metastatic breast cells （MDA- MB-231 ） and low metastatic breast cells （MCF- 7 ） were investigated. The capability of cell migration was identified by Scratch Test. Real -time PCR and Western blot were used to measure the mRNA and protein expression of STIM1, respectively. SOCE were detected by laser confocal microscopy. Results The numbers of migrated MCF -7 cells at 24 h and 48 h were significantly lower than those of migrated MDA - MB - 231 cells （ P 〈 0. 01 ）. Both mRNA and pro- tein expression of STIM1 in MCF - 7 ceils （ 1. 001 9 ± 0. 074 4 and 1. 296 8± 0. 075 8, respectively） was significantly higher than that in MDA - MB -231 cells （6. 338 8±1. 046 7 and 0. 530 2±0. 177 8, respectively）. Furthermore, the Ca2＋ concentration caused by SOCE in MDA - MB - 231 cells （ AF/F0 = 1. 036 3±0. 413 5 ） was significantly higher than that in MCF - 7 cells （ AF/FO = 1. 036 3±0. 413 5 ; P 〈 0. 01 ）. Conclusion Higher STIM1 expression and calci- um influx are found in high metastatic breast cells, which probably involved in metastasis.
出处
《广东医学》
CAS
CSCD
北大核心
2013年第1期31-33,共3页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:30971193)
