摘要
目的:建立同时测定布渣叶中牡荆苷和异牡荆苷含量的方法。方法:采用反相高效液相色谱法。色谱柱为SHISEIDO Capcell pak MG C18(250mm×4.6mm,5μm),流动相为甲醇-0.4%甲酸溶液(30:70,V/V),流速为1.0ml/min,检测波长为339nm,柱温为30℃。结果:牡荆苷和异牡荆苷的进样量分别在53.40～1334.00、73.90～1847.00ng范围内与各自峰面积积分值呈良好线性关系(r均为0.9999);平均加样回收率分别为99.00%和99.37%,RSD分别为1.87%和1.77%(n均为9)。结论:本方法重复性好、操作简便、结果准确可靠,可为布渣叶的质量评价和资源利用提供试验依据。
OBJECTIVE： To develop a method for simultaneous determination of vitexin and isovitexin in Microcos paniculata. METHODS： RP-HPLC method was adopted. The determination was performed on SHISEIDO Capcell pak MG C18 （250 mm× 4.6 mm, 5 μm） with mobile phase composed of methanol-0.4% formic acid （30 ： 70, V/V） at the flow rate of 1.0 ml/min. The detection wavelength was set at 339 nm and the column temperature was maintained at 30 ℃. RESULTS： The linear ranges of vitexin and isovitexin were 53.40-1 334.00 ng（r=0.999 9） and 73.90-1 847.00 ng（r=0.999 9）. The average recoveries were 99.00% （RSD= 1.87%, n=9） and 99.37% （RSD= 1.77%, n=9）. CONCLUSION： The method is reproducible, simple and accurate, and it pro- vides reference for quality evaluation and resource application of M. paniculata.
出处
《中国药房》
CAS
CSCD
2013年第7期630-633,共4页
China Pharmacy
基金
广东省中医药局建设中医药强省课题资助(No.20111131)
