摘要
目的:研究玫瑰花露(RW)抑制紫外线A和B诱导永生化角质形成细胞株(HaCaT)凋亡的分子机制。方法:复制紫外线A和B辐射诱导的HaCaT凋亡的模型,实验分为分6组,即对照、模型、阳性对照[维生素C(5.68mmol/L)或N-乙酰半胱氨酸(5mmol/L)]和RW高、中、低浓度(0.2%、0.1%、0.05%)组。采用MTT法检测RW对HaCaT细胞活性的影响;流式细胞术检测细胞凋亡率及细胞内活性氧(ROS)水平;酶化学法测定细胞超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)水平和丙二醛(MDA)含量;Western blot法检测HaCaT细胞IκBα磷酸化水平。结果:与模型组比较,RW高、中浓度组HaCaT细胞活性显著增强,细胞凋亡率显著降低,细胞内ROS水平显著降低,SOD、GSH-Px、CAT活性和T-AOC水平显著升高,MDA含量显著降低(P<0.01或P<0.05);RW高、中、低浓度组HaCaT细胞IκBα磷酸化表达显著降低(P<0.01)。结论:RW可通过调节核转录因子(NF)-κB通路抑制紫外线诱导的HaCaT细胞凋亡。
OBJECTIVE： To investigate the inhibition mechanism of rose water （RW） on UVA and UVB-induced apoptosis of HaCaT cells. METHODS ： The apoptotic model of HaCaT cells was induced by UVA and UVB. Experiment design was divided in- to 6 groups： control group, model group, positive control[vitamin C（5.68 mmol/L or NAC（5 mmol/L）] group, RW high-concentra- tion, RW medium-concentration, RW low-concentration groups（0.2%, 0.1%, 0.05% ）. The cell activity was detected by MTT; flow cytometric analysis was used to investigate the cellular apoptosis rate and the ROS level; zymochemistry method was performed to detect the activity of SOD, GSH-Px, CAT and T-AOC levels, MDA content; the phosphorylation of IκBa was assayed by using Western blot analysis. RESULTS：Compared with model group, HaCaT cell activity was enhanced significantly, The percentage of apoptotic cells was reduced significantly, intracellular ROS levels were decreased significantly, SOD, GSH-Px, CAT activity and T-AOC levels were increased significantly, the content of MDA was decreased significantly in RW high-concentration and RW medi- um-concentration groups（P〈0.01 or P〈0.05）. p-IκBa expression was reduced significantly in RW high-concentration, RW medi- um-concentration and RW low-concentration groups （P〈0.01）. CONCLUSION： RW inhibit the UV-induced apoptosis of HaCaT cells by regulating NF-κB pathway.
出处
《中国药房》
CAS
CSCD
2013年第7期592-595,共4页
China Pharmacy
基金
青岛市科技局科研项目[No.11-2-4-2(-1)-jch]
