摘要
In the past two decades, two-photon microscopy （TPM） transforms biomedicalresearch, allowing non- destructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we review the recent advances of TPM technologyincluding novel laser sources, new image acquisition paradiams, and microendoscopicimaging systems. Then, we survey the capabilities of TPM imagingof biological relevant molecules such as nicotinamideadenine dinucleotide （NADH）, flavin adenine dinucleotide （FAD）,and reactive oxygen species （ROS）. Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.
In the past two decades, two-photon microscopy （TPM） transforms biomedicalresearch, allowing non- destructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we review the recent advances of TPM technologyincluding novel laser sources, new image acquisition paradiams, and microendoscopicimaging systems. Then, we survey the capabilities of TPM imagingof biological relevant molecules such as nicotinamideadenine dinucleotide （NADH）, flavin adenine dinucleotide （FAD）,and reactive oxygen species （ROS）. Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.
基金
H. Guo andY. Chen are supported by National Institutes of Health(NIH)under Grant Nos.R21AG042700,R21DK088066,and R21EB012215
L. Qin and S. Cho are supported by NIH under Grant No.R01HL082511
H. Aleyasin and R. R. Ratan are supported by NIH under Grant Nos.P01AG14930-10,NS04059,and NS39170
W. Gong, J.Chen, and S. Xie are supported by the National Natural Science Foundation of China under Grant No.60678054
