摘要
目的:体外原代培养脂肪源性干细胞(adipose-derived stem cell,ADSC),并在细胞水平检测各种神经营养因子(neurotrophic factor,NF)的表达情况,探讨ADSC是否可以作为神经损伤修复的种子细胞。方法:体外原代培养和鉴定ADSC,应用RT-PCR方法检测ADSC细胞神经营养因子,包括神经生长因子(nerve growth factor,NGF)、脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)、神经营养素-3(neurotrophin 3,NT-3);免疫组织化学法检测粘附因子(nerve cell adhesion molecule,NCAM)表达情况。结果:ADSC可以向脂肪和骨两方面分化;RT-PCR结果显示ADSC细胞NGF、BDNF、NT-3 mRNA水平明显升高;同时免疫荧光法检测到了高水平的NCAM。结论:ADSC具有干细胞分化潜能,并且能够表达NF和NCAM。
Objective： Adipose-derived stem cells （ADSCs） were isolated from rat adipose tissue and cultured in vitro, and the expression of various neurotrophic factors （NF） at cellular level was examined, aiming to investigate whether AD- SCs could serve as seed cells to improve the peripheral nerve in jinx. Methods ： Isolation, culture and identification of AD- SCs were conducted in vitro and; polymerase chain reaction （RT-PCR） was used to detect the expression of neurotrophic factors, including nerve growth factor （NGF） , brain-derived neurotrophic factor （BDNF） and neurotrophin 3 （NT-3） ; Immunocytochemistry was used to detect the expression of nerve cell adhesion molecule （NCAM）. Results： ADSCs showed adipogenic and osteogenic potential; RT-PCR results showed the level of mRNAs of NGF, BDNF and NT-3 in- creased significally ; and higher protein expression level of NCAM was detected in ADSCs by immunohistochemistry. Con- clusion ： ADSCs expressed neurotrophic factors and NCAM, indicating that it possessed the differentiation potential of stem cells.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2013年第1期61-64,共4页
Chinese Journal of Neuroanatomy
基金
黑龙江省2011年研究生创新科研资金项目YJSCX2011-299HLJ
关键词
脂肪源性干细胞
神经再生
神经营养因子
adipose-derived stem cells （ADSCs）
nerve regeneration
neurotrophic factors（NFs）
