摘要
[目的]观察中医骨折三期治疗在家兔骨折模型愈合过程中,转化生长因子β(TGF-β)与碱性成纤维细胞生长因子(bFGF)表达的影响,探讨骨折中药分期论治的作用机制。[方法]选用174只成年健康雄性新西兰家兔,均建立桡骨远端骨折模型,利用计算机产生随机数字的方法,174只家兔随机取出6只作为正常对准组,其余分为四组,分别为中药三期治疗组、中药二期治疗组、中药一期治疗组、模型对照组,每组42只。每组又分为3、6、9、14、28、42、56 d 7个时间点,每个时间点6只,三期组分早、中、晚期分别予加减肢伤一方、二方与三方治疗,二期组早期予肢伤一方;后期予肢伤二方与三方的综合方治疗,一期组前三方综合方治疗,正常对准组和模型组给予等量蒸馏水。每组于不同时间点分别处死6只,取材,常规组织学检查,免疫组化法检测标本中的TGF-β与bFGF,应用计算机图像分析系统进行定量分析。[结果]各中药治疗组对于骨折端外骨膜、内骨膜和骨髓内组织TGF-β与bFGF表达平均阳性细胞平均光密度(MOD)值:二期组>三期组>一期组>模型组(P<0.05)。[结论]中药治疗能显著促进骨折愈合过程中TGF-β与bFGF的表达,二期治疗明显强于三期治疗、一期治疗,骨折分期辨证治疗的最佳分期为二期治疗。
[ Objective ] To observe the effects of the different period treatment theory of bone fracture in TCM （ Traditional Chinese Medicine）on TGF-β and bFGF expression in the issues of outer periosteum, endosteum and bone marrow of rabbits, and to explore the rationality of phasing method in TCM in treating fracture, and which is the best method. [ Method ] 3 mm bone fracture line were made at lower one third part of both radius in 174 male healthy rabbits. Six rabbits were randomly taken into nomal group from these rabbits, and others were randomly divided into four groups, every group had 42 rabbits, including three period treatment group （ TTG）, two period treatment group （ TTG）, one period treatment group （ OTG）, model control group （MCG）. TTG were treated with three period treatment, two period treatment for WTG, and OTG were treated with one period treatment,rabbits in MCG were given no prescription or drug but distilled water as same dose as that of other groups. At day 3, 6,9,14,28,42 and 56, six rabbits from every group were randomly selected and were killed by aeroembolism. The left radiuses were taken out as the research object. Immunohistochemistry stain was performed to examinate the TGF-β and bFGF expression in the outer periosteum,endosteum and bone marrow. [ Result] The TGF-β and bFGF expression of all TCM treatment groups were enhanced in the outer periosteum, endosteum and bone marrow at different time points in fracture healing. The TGF-β and bFGF expression in the two tissues of TYG had the tendency of higher than that of the other groups at the most time points after operation. [ Conclusion] Treating fracture in stages has more predominant effect on the expression of TGF-β and bFGF than thatof treating fracture with single prescription or drug. The period treatment for fracture is very necessary, and the two period treatment is the best method.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2013年第4期381-386,共6页
Orthopedic Journal of China
基金
国家自然基金资助项目(编号:30973762)
关键词
骨折
转化生长因子Β
碱性成纤维细胞生长因子
免疫组化
兔
fractures, transforming growth factor beta, basic fibroblast growth factor, immunohistochemistry,rabbits
