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实时荧光定量PCR检测转基因阿尔茨海默病小鼠脑组织中miRNAs的差异表达 被引量：8

Real-time PCR for detecting differential expressions of microRNAs in the brain of a transgenic mouse model of Alzheimer's disease
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摘要 目的通过实时荧光定量PCR方法检测APPswe/PS△E9双转基因小鼠和对照小鼠脑组织中miRNA-135a-5p、miRNA-135a-2-3p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p的表达。方法选取6月龄大小的APPswe/PS△E9双转基因小鼠为实验组,同月龄同种系的野生型小鼠C57为对照组,分别提取其脑组织总miRNA,应用实时荧光定量PCR方法检测两组小鼠脑组织中miRNA-135a-5p、miRNA-135a-2-3p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p的表达。结果上述5种miRNAs在实验组与对照组小鼠中的相对表达量分别为0.73±0.27、1.08±0.58,2.47±6.15、1.65±0.67,0.72±0.14、1.31±0.73,0.57±0.34、1.06±0.35,0.63±0.26、1.02±0.18。其中有统计学意义的是miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p(P≤0.05)。结论 miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p在APPswe/PS△E9双转基因小鼠中有差异表达,可能在阿尔茨海默病的发病过程中起着重要作用。 Objective To detect the expression of miRNA-135a-5p, miRNA-135a-2-3p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p in the brain tissue of the APPswe/PS △ E9 double transgenic mouse model of Alzhaimer＇s disease using real-time PCR. Methods Six-month-old APPswe/PS △ E9 double transgenic mice and wild-type C57 mice of the same species were examined for the expressions of miRNA-135a-5p, miRNA-135a-2-3p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p in the brain tissue using real-time PCR. Results The relative expression levels of the 5 miRNAs in the transgenic versus the wild-type mice were 0.73±0.27 vs 1.08±0.58, 2.47±6.15 vs 1.65±0.67, 0.72±0.14 vs 1.31±0.73, 0.57±0.34 vs 1.06±0.35, and 0.63＋0.26 vs 1.02±0.18, respectively,showing sign/ficance differences in the expressions of miRNA-135a-Sp, miRNA-298-Sp, miRNA-466b-3p, and miR-669f-3p between the two groups （P〈0.05）. Conclusion miRNA-135a-5p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p are expressed differentially in APPswe/PS △ E9 double transgenic mice, suggesting their important roles in the pathogenesis of Alzheimer disease.
作者 田密 丁宇 侯德仁 邓炎尧 李维 奉夏露
机构地区 中南大学湘雅三医院神经内科
出处 《南方医科大学学报》 CAS CSCD 北大核心 2013年第2期262-266,共5页 Journal of Southern Medical University
基金 湖南省科技厅社会发展支撑计划课题(009SK3175 2012SK3218)
关键词 阿尔茨海默病 转基因阿尔茨海默病小鼠 miRNA 实时荧光定量PCR Alzheimer disease transgenic mice microRNA real-time polymerase chain reaction
分类号 R749.16 [医药卫生—神经病学与精神病学]
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参考文献18

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共引文献4

同被引文献66

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引证文献8

二级引证文献26

南方医科大学学报
2013年 第2期

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